LKEK10081U Enzymology and Experimental Biochemistry
MSc Programme in Biology-Biotechnology
BSc Programme in Chemistry
The course comprises the theoretical and experimental work on
enzymes, proteins and peptides in biological matrices. Focus is
placed on experimental and theoretical understanding of the
relations between structure and properties of the molecules. Topics
* Extraction techniques
* Chromatographic systems
* Solvent and buffer relations
where effects of ions, organic molecules, and surfactants play a significant role.
The combinations of such knowledge give the students basis for understanding and development of a relevant strategy of methods as used in analytical biochemistry. The students will in selected projects work with both traditional and new actual /advanced biochemical methods and techniques.
Strategies to efficient isolation, purification and characterisation of biomolecules, bioactive proteins, peptides, and enzymes from complex biological systems
Determination of kinetic parameters, Vmax, KM and kcat, and interpretation of such data in relation to ideal catalytic effects
Thermodynamics and kinetics in enzymology and biochemical analyses
Binding between molecules in equilibrium reactions and thermodynamic stable complexes
Receptor and effector sites, positive (activators) and negative effectors (inhibitors) and their effects on the active sites
Native enzymes: holoenzymes, apoenzymes, co-factors, activation energy and kinetics
Design and use of enzymatic assays
A project with individual final reporting is a part of the course where the students in a group of 2-4 persons perform purification of a selected enzyme or inhibitor.
The course provides a thorough and fundamental theoretical and
practical basis for knowledge to and work with biomolecules,
especially bioactive proteins, peptides, and enzymes occurring in
complex biological matrices, plant, animal and microbial based feed
and food. Focus is placed on the relations between structure and
properties of biomolecules, strategies in use of actual new and
traditional biochemical methods and understanding of the
descriptions in original scientific papers.
After completing the course the student should be able to:
- Understand physico-chemical-biochemical properties of biomolcules and especially enzymes.
- Describe strategies to efficient isolation and purification of enzymes from complex biological systems
- Describe analytical techniques used in biochemistry and for enzyme characterisation.
- Understand the theoretical basis for changes of water as solvent, extraction and chromatographic systems in work with molecules of different polarity.
- Evaluate the detection, quantification and linearity of experimental data in relation to published values (literature data).
- Design experimental stategies in analytical biochemistry and enzymology based on structure and properties of analytes in complex matrix systems.
- Apply theoretical principles of analytical biochemistry to carry out experimental isolation and purification of enzymes
- Evaluate the performance of applied techniques in an enzyme related project carried out in a group (preferably 2-4 persons).
- Cooperate with a fellow student on laboratory reports and a project report
- Expand the knowledge to biochemistry by use of mathematics evaluations
- Utilise the obtained knowledge in more comprehensive projects within research, development or industry.
See Absalon for a list of course literature
- 7,5 ECTS
- Type of assessment
- Oral examination, 20 minEvaluation of project report supplemented with individual oral presentation and examination in curriculum (no preparation time).
Weight: Oral presentation of project report and subsequent examination in the report and curriculum are 100% of the final grade.
- Exam registration requirements
Submission and approval of at least 75% of reports of performed experiments. Submission of individual project report.
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
Several internal examiners
Same as ordinary exam.
If the exam registration requirements have not been met, reports of performed experiments and individuel project report must be submitted and approved no later than two weeks before the re-exam.
Criteria for exam assesment
See Learning Outcome
- Theory exercises
- Practical exercises