LKEK10081U Enzymology and Experimental Biochemistry
BSc Programme in Chemistry
The course comprises the theoretical and experimental work on
enzymes, proteins and peptides in biological matrices. Focus is
placed on experimental and theoretical understanding of the
relations between structure and properties of the molecules. Topics
includes are:
* Extraction techniques
* Chromatographic systems
* Solvent and buffer relations
where effects of ions, organic molecules, and surfactants play a
significant role.
The combinations of such knowledge give the students basis for
understanding and development of a relevant strategy of methods as
used in analytical biochemistry. The students will in selected
projects work with both traditional and new actual /advanced
biochemical methods and techniques.
Strategies to efficient isolation, purification and
characterisation of biomolecules, bioactive proteins, peptides, and
enzymes from complex biological systems
Determination of kinetic parameters, Vmax, KM and kcat, and
interpretation of such data in relation to ideal catalytic effects
Thermodynamics and kinetics in enzymology and biochemical analyses
Binding between molecules in equilibrium reactions and
thermodynamic stable complexes
Receptor and effector sites, positive (activators) and negative
effectors (inhibitors) and their effects on the active sites
Native enzymes: holoenzymes, apoenzymes, co-factors, activation
energy and kinetics
Design and use of enzymatic assays
A project with individual final reporting is a part of the course
where the students in a group of 2-4 persons perform purification
of a selected enzyme or inhibitor.
The course provides a thorough and fundamental theoretical and
practical basis for knowledge to and work with biomolecules,
especially bioactive proteins, peptides, and enzymes occurring in
complex biological matrices, plant, animal and microbial based feed
and food. Focus is placed on the relations between structure and
properties of biomolecules, strategies in use of actual new and
traditional biochemical methods and understanding of the
descriptions in original scientific papers.
After completing the course the student should be able to:
Knowledge:
Reflect about physico-chemical-biochemical properties of
biomolcules and especially enzymes.
Describe strategies to efficient isolation and purification of
enzymes from complex biological systems
Describe analytical techniques used in biochemistry and for enzyme
characterisation.
Understand the theoretical basis for changes of water as solvent,
extraction and chromatographic systems in work with molecules of
different polarity.
Skills
Evaluate the detection, quantification and linearity of
experimental data in relation to published values (literature
data).
Design experimental stategies in analytical biochemistry and
enzymology based on structure and properties of analytes in complex
matrix systems.
Apply theoretical principles of analytical biochemistry to carry
out experimental isolation and purification of enzymes
Evaluate the performance of applied techniques in an enzyme related
project carried out in a group (preferably 2-4 persons).
Competences
Cooperate with a fellow student on laboratory reports and a project
report
Expand the knowledge to biochemistry by use of mathematics
evaluations
Utilise the obtained knowledge in more comprehensive projects
within research, development or industry.
Collection of book chapters and selected original scientific literature relevant for projects. More information on Absalon prior to the course start.
- Category
- Hours
- Exam
- 1
- Lectures
- 24
- Practical exercises
- 72
- Preparation
- 95
- Theory exercises
- 14
- Total
- 206
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- Credit
- 7,5 ECTS
- Type of assessment
- Written assignmentOral examination, 30 minEvaluation of reports of performed experiments supplemented with individual oral presentation and examination
Weight: Oral examination in project report and curriculum is judged by 100 % - Exam registration requirements
- Submission and approval of at least 75 % of project reports
- Aid
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
More than one internal examiner
Criteria for exam assesment
Fullfilment of learning outcome
Course information
- Language
- English
- Course code
- LKEK10081U
- Credit
- 7,5 ECTS
- Level
- Full Degree Master
- Duration
- 1 block
- Placement
- Block 2
- Schedule
- A (Tues 8-12 + Thurs 8-17)
- Course capacity
- The number of students per course is limited to 40 owing to limited space in the laboratories and the teaching in use of advanced instrumentation.
- Continuing and further education
- Study board
- Study Board of Biomolecular Sciences and Technology
Contracting department
- Department of Food Science
Course responsibles
- Jens Christian Sørensen (4-7b74798451778080753f7c863f757c)
- Keld Ejdrup Markedal (3-7b757150767f7f743e7b853e747b)