SLVKA0361U Pharmaceutical Analytical Chemistry

Study groups and exercises constitute an integrated course package. The course provides a background for selecting and developing analytical methods and assessing the reliability of analytical data. A variety of analytical techniques are described with regard to their use in quantitative determination and identification. Teaching will focus on the design and use of apparatus as well as potential sources of error. There will also be focus on quality assurance and validation of analytical methods, including the significance of linearity, accuracy, precision, sensitivity, determination limits, blind values, selectivity and interference. Study groups are used to discuss theoretical background. The students take turns giving 30-minute presentations on theoretical subjects, which are subsequently discussed and related to the practical experiments. In the practical exercises, students work with fixed demonstrations of separation and detection methods. They simultaneously work on a project where they plan and try out a number of these techniques on a given drug. The analytical techniques are liquid chromatography (HPLC), gas chromatography (GC), capillary electrophoresis (CE), UV-VIS spectrophotometry, fluorometry, mass spectrometry (MS), atomic absorption spectrometry (AAS) and potentiometric methods. The students carry out experiments in groups and submit a joint report. Reports are assessed by the teacher and discussed on return.