SBRI19011U Translational Tools II – New Methods
BRIDGE - Translational Excellence Programme
The main themes covered in the course include:
- Stem cells and induced pluripotent stem cells, and their applications in disease modeling
- Precision nuclease-based genome editing
- Proteolysis targeting chimera (PROTACs)
- Mass spectrometry for understanding drug mechanisms
- High resolution microscopy
- Cryo-EM
Upon completing the course, participants should be able to:
Knowledge
Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas:
- Gene editing
- PROTACs
- Cryo-EM
- Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery.
Skills
- Culture and use mouse embryonic stem cells
- Design of constructs for CRISPR-based gene targeting
- Identifying targets of PROTACs using mass spectrometry
- Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins.
Competences
- Identify and evaluate advantages and limitations of the use of above listed methods.
- Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
- Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public.
Course literature will be published on Absalon.
In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.
The course will end with an evaluation where participants must reflect on course learning outcomes and give feedback for course development.
- Category
- Hours
- Lectures
- 10
- Preparation
- 5
- Theory exercises
- 5
- Exercises
- 20
- Total
- 40
The BRIDGE – Translational Excellence Programme offers a few selected graduated PhDs a two-year postdoctoral fellowship in translational medicine. This course is only available to the fellows enrolled in the programme. Fellows are automatically enrolled in this course upon admission to the programme.
For further information about the programme, please go to the website: www.bridge.ku.dk
- Credit
- 0 ECTS
- Type of assessment
- Continuous assessmentRequirement to attend classes
- Type of assessment details
- Attendance and active participation.
- Exam registration requirements
Participants are automatically registered for the examination upon admission to the BRIDGE - Translational Excellence Programme.
- Aid
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assesment
Active contribution and course participation according to the BRIDGE Guidelines and Practicalities.
Course information
- Language
- English
- Course code
- SBRI19011U
- Credit
- 0 ECTS
- Level
- Part Time MasterPh.D.
- Placement
- Autumn
- Schedule
- See course dates and course programme in Absalon.
*This course is offered in autumn 2025 for the BRIDGE 2024-2026 Academic Curriculum. - Course capacity
- 15 participants
Study board
- Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
Contracting department
- Center for Protein Research
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinators
- Chuna Ram Choudhary (chuna.choudhary@cpr.ku.dk)
- Takeo Narita (takeo.narita@cpr.ku.dk)
- Moreno Papetti (moreno.papetti@cpr.ku.dk)