SBRI19011U Translational Tools II – New Methods

Volume 2024/2025
Education

BRIDGE - Translational Excellence Programme

Content

The main themes covered in the course include:

  • Stem cells and induced pluripotent stem cells, and their applications in disease modeling
  • Precision nuclease-based genome editing
  • Proteolysis targeting chimera (PROTACs)
  • Mass spectrometry for understanding drug mechanisms
  • High resolution microscopy
  • Cryo-EM
Learning Outcome

Upon completing the course, participants should be able to:
 

Knowledge

Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas:

  • Gene editing
  • PROTACs
  • Cryo-EM
  • Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery.

 

Skills

  • Culture and use mouse embryonic stem cells
  • Design of constructs for CRISPR-based gene targeting
  • Identifying targets of PROTACs using mass spectrometry
  • Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins.

 

Competences

  • Identify and evaluate advantages and limitations of the use of above listed methods.
  • Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
  • Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public.
Literature

Course literature will be published on Absalon.

Participants must meet the admission criteria of the BRIDGE - Translational Excellence Programme.
The course will be organized with a mix of scientific seminars by invited speakers including lectures about stem cells, PROTACs, CRISPR-based genome engineering, high-resolution microscopy, cryo-EM, as well as the applications of modern proteomics technologies for translational research.

In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.

The course will end with an evaluation where participants must reflect on course learning outcomes and give feedback for course development.
  • Category
  • Hours
  • Lectures
  • 10
  • Preparation
  • 5
  • Theory exercises
  • 5
  • Exercises
  • 20
  • Total
  • 40
Oral
Continuous feedback during the course of the semester
Credit
0 ECTS
Type of assessment
Continuous assessment
Requirement to attend classes
Type of assessment details
Attendance and active participation.
Exam registration requirements

Participants are automatically registered for the examination upon admission to the BRIDGE - Translational Excellence Programme.

Aid
All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assesment

Active contribution and course participation according to the BRIDGE Guidelines and Practicalities.