SBIB10170U Mammalian Genomics
BSc Programme in Biology-Biotechnology
BSc Programme in Natural Sciences and IT
BSc Programme in Biology
MSc Programme in Animal-derived Foods (Food of Life)
The course is an advanced course in theoretical and practical
genomics with focus on mammals. The course will provide information
that will allow the student to gain knowledge on methods and
technologies currently used to dissect, describe and characterize
complex genomes. Aspects of both research and application within
the field of genomics will be addressed.
The students will also learn innovative thinking.
* The theoretical part of the course will cover the following
topics:
1) Genome projects, Model organisms & Comparative genomics
2) Organization and content of the mammalian genome
3) Human genetic variability
4) Genetic mapping of mendelian characters
5) Mapping genes conferring susceptibility to complex diseases
6) Gene expression
7) Sequencing genomes: techniques, challenges and bioinformatic
analysis
8) Molecular pathology/Cancer/Pharmacogenetics
9) Genetic testing of individuals
10) Genetic manipulation of cells and animals. Gene therapy
11) MicroRNAs
*A case-work based on innovation will be held during the
course
* Virtual-lab exercises (computer work): Labster and UCSC browser
sessions will be held during the course
* The practical part of the course will contain:
1) DNA forensics /paternity testing (lecture/lab work)
2) RNA isolation /qPCR (lab work)
More information will be given at the beginning of the
course.
After completion of the course the student is expected to have:
Knowledge:
- Describe the architecture and components of an average mammalian
genome.
- Understand the possibilities of analyzing and characterizing
mammalian genomes.
- Understand qualitative and quantitative genetics with focus on
mammalian disease genetics.
- Describe the technologies and methods involved in genome projects
and the objectives of the different genome projects.
- Understand how comparative genomics can be used in the
characterization and annotation of genomes by transferring
genomic information from one species to another (comparative
genomics).
- Understand the regulation of gene expression at different
levels and how to study it. Focus on qPCR.
- Understand NGS technique
- Understand how mutations can cause inherited defects and
predisposition to disease such as cancer and the possibilities and
relevance of genetic testing.
- Understand innovation.
Skills:
- Use of basic and advanced methods in molecular genetics.
- Perform DNA sequence analysis, simple sequence assembly and
annotation and detect sequence variation by using the UCSC browser.
- Perform PCR and microsatellites analysis.
- Perform qPCR and analyse expression profiles of genes in
different tissues.
Competences:
- Evaluation of strategies to dissect the genetics underlying
inherited traits in mammals.
- Evaluate the practical use of genetic testing.
- Think more innovative in relation to
Biology-Biotechnology.
Information about teaching material will be available at Absalon before the course starts
Crash-bioinformatic session
Quizzes after some lectures
- Category
- Hours
- E-Learning
- 3
- Exam
- 0,5
- Lectures
- 43
- Practical exercises
- 20
- Preparation
- 117,5
- Project work
- 10
- Theory exercises
- 12
- Total
- 206,0
Biology-Biotechnology students have priority over the other educations.
Open for credit transfer students. Apply here:
http://healthsciences.ku.dk/education/other-programme-options/credit-transfer-students/
- Credit
- 7,5 ECTS
- Type of assessment
- Oral examination, 20 minutesThe 20 min. oral examination is based on the practical laboratory work and on the curriculum.
The student should show adequate knowledge and understanding of both practical and curriculum related subjects in order to pass the exam.
Weight: Oral examination 100 %. - Exam registration requirements
An approved written report about the practicals is pre-requisite for access to oral examination and/or re-examination.
- Aid
- Only certain aids allowed
The students can bring their own notes.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
2 examinators
- Exam period
Week 25. The exact days will be anounced at the beginning of the course.
- Re-exam
An approved written report about the practicals is pre-requisite for access to oral re-examination.
Criteria for exam assesment
To achieve the maximum grade of 12, the shall be able to:
Knowledge:
- Describe the architecture and components of an average mammalian
genome.
- Understand the possibilities of analyzing and characterizing
mammalian genomes, and to understand qualitative and quantitative
genetics with focus on mammalian disease genetics.
- Describe the technologies and methods involved in genome
projects and the objectives of the different genome projects.
- Understand how comparative genomics can be used in the
characterization of genomes by transfering genomic information from
one species to another.
- Understand the regulation of gene expression on different levels
and which techniques to apply to study it. Special
focus on NGS and qPCR.
- Understand how mutations can cause inherited defects and
predisposition to disease such as cancer and the possibilities and
relevance of genetic testing.
Skills:
- Knowledge on how to perfom basic and advanced methods in
molecular genetics.
- Knowledge on how to perfom DNA sequence analysis, simple sequence
assembly and annotation and detect sequence variation by using the
UCSC browser.
- Knowledge on how to perfom PCR.
- Knowledge on how to perfom qPCR and analyse expression profiles
of genes in different tissues.
Competences:
- Evaluation of strategies to dissect the genetics underlying
inherited traits in mammals.
- Evaluate the practical use of genetic testing.
- Think more innovative in relation to Biology-Biotechnology.
In order to complete the course with the grade 12, the student should give an excellent performance during the exam displaying a high level of command of all aspects of the drawn practical and theoretical subjects. The student should show an exhaustive comprehension of all the matters/principles asked during the exam with no or only few minor mistakes/weaknesses, and he/she should be able to integrate knowledge from different subjects and areas when relevant.
Course information
- Language
- English
- Course code
- SBIB10170U
- Credit
- 7,5 ECTS
- Level
- BachelorFull Degree Master,Part Time Master,Ph.D.
- Duration
- 1 block
- Placement
- Block 4
- Schedule
- B
- Course capacity
- 60 participants
- Continuing and further education
- Study board
- Study Board for the Biological Area
Contracting department
- Department of Veterinary and Animal Sciences
Course Coordinators
- Susanna Cirera (3-79697946797b746a34717b346a71)
Lecturers
Intern and extern teachers