NSCPHD1180 Advanced Chromatography and Mass Spectrometry

Volume 2013/2014
Content
This course shares lectures with the MSc course "Advanced Analytical Chemistry - Chromatography and Mass Spectrometry - NPLK13004U

Advanced course in chromatography and mass spectrometry for chemical analysis of organic compounds. This course is a natural extension of any basic course in analytical chemistry for everybody who is going to use modern hyphenated techniques such as GC-MS, LC-MS(MS) for analysis of complex mixtures. The course covers chromatographic separation of organic compounds with special emphasize on the molecular mechanism and theory of analyte-column interactions for liquid and gas chromatography, and the theory of ionization, fragmentation, mass separation, detection and data-analysis for all kind of mass spectrometers and ionization techniques in common use coupled to gas chromatography, liquid chromatography and capillary electrophoresis. All common analytical instruments and techniques from GC-FID/MS to UPLC-UV/FLU/MSMS are addressed. The theory is supplemented with computer simulations and hands-on experiments and expert guest-lectures of selected topics.
Learning Outcome
This course enables the student to plan and perform analysis of complex mixtures using modern chromatographic techniques and high-end detectors, including the selection of method, columns and mode of detection, as well as optimization of chromatographic and mass spectrometric conditions for all common instrumental analytical separation methods.

"Introduction to Modern Liquid Chromatography", by Lloyd R. Snyder, Douglas Kirkland and John W. Dolan, Wiley 2009, and "Mass Spectrometry. Principles and Applications", by Edmond de Hoffmann and Vincent Stroobant, 3rd Ed., Wiley 2007. "High-Performance Gradient Elution. The Practical Application of the Linear-Solvent-Strength Model", by Lloyd R. Snyder and John W. Dolan, Wiley 2007 and “Interpretation of Mass Spectra”, by Fred W. McLafferty and Frantisek Turecek, University Science Books 1993 will be used to some extend. Furthermore, selected articles will be included.

As prerequisite a course in instrumental analysis, like the course "Analytical Chemistry", is strongly recommended, since all basic theory of separation, chromatographic instruments and mass spectrometry is assumed to be known.
For questions feel free to contact Nikoline Juul Nielsen or Bo Svensmark.
Lectures, student presentations of curriculum, expert lectures, excursions, hands-on experiments, computer simulations, and individual project related to your PhD.
  • Category
  • Hours
  • Exam
  • 10
  • Lectures
  • 48
  • Practical exercises
  • 16
  • Preparation
  • 120
  • Theory exercises
  • 16
  • Total
  • 210
Credit
7,5 ECTS
Type of assessment
Oral examination
Student presentations of curriculum are mandatory.
Exam registration requirements
4 ECTS is given if students follew the course in either Chromatography only or Mass Spectrometry only.