NPLK13003U Advanced Analytical Chemistry - Sampling and Sample Preparation
MSc Programme in Biotechnology
MSc Programme in Chemistry
MSc Programme in Environmental Science
Sampling and sample preparation is a cornerstone in performing
sound experiments, and thus essential to any kind of analytical
chemistry project: the results obtained will never be better than
the samples injected.
The course starts with a thorough treatment of Pierre Gy’s Theory
of Sampling (ToS), which tells how to obtain a representative
sample without systematic errors and with an acceptable
uncertainty. The practice and tools for taking the samples
and reduce the sample mass in a representative way is treated based
on ToS. All common matrices, soil, water and air, are
addressed.
The second part of the course covers the sample preparation
techniques for extraction, purification and pre-concentration of
samples for injection into modern analytical instruments such as GC
and, HPLC. This part of the course includes: active and passive
sampling for gases (adsorbents, traps, filtering etc.), sample
preparation for liquid matrices (Liquid/Liquid Extraction – LLE,
Solid Phase Extraction – SPE, Solid Phase Micro-Extraction – SPME,
Stir-Bar Solid Extraction – SBSE, Supported Liquid Extraction – SLE
etc.), for solid matrices (shake, Microwave Assisted Extraction –
MAE, Ultrasonication, Pressurised Liquid Extraction – PLE,
Supercritical Fluid Extraction – SFE, etc.), and various
headspace methods.
The theory is supplemented with computer simulations, laboratory
experiments, a field exercise and expert guest-lectures of
selected topics.
This course enables the student to plan and perform sampling and sample preparation, including sampling strategy, determination of number of samples and sample size, sample selection, sampling techniques and tools, and methods for sample preparation for organic analytes, extraction, filtering, purification and pre-concentration including advanced methods like SPME, SBSE, PLE, SLE for common instrumental analytical methods.
Knowledge:
Describe the theory and principles for representative sampling according to Gy’s Theory of Sampling. Describe the theory for active and passive sampling. Describe the theory, tools, instruments, and techniques for sample preparation of organic analytes.
Skills:
Plan sampling experiments and analyse the results in terms of sampling variance using ANOVA and variographic analysis. Predict sampling variance using Gy’s formula for simple matrices. Plan sample preparation including selection of tools, techniques and how to optimize sample preparation procedure for organic analytes.
Competences:
Plan and perform sampling pilot experiments and real sampling for chemical analysis. Describe, develop and apply sample preparation methods for instrumental analysis of organic compounds. Evaluate and discuss the scientific literature within sampling and sample preparation.
Notes and selected peer-reviewed papers will be used extensively.
Textbook: "Handbook of Sample Preparation" ed. Janusz Pawliszyn & Heather L. Lord (Available on Rex)
Academic qualifications equivalent to a BSc degree is recommended.
- Category
- Hours
- Lectures
- 26
- Class Instruction
- 12
- Preparation
- 106
- Theory exercises
- 20
- Practical exercises
- 12
- Excursions
- 10
- Exam
- 20
- Total
- 206
As
an exchange, guest and credit student - click here!
Continuing Education - click here!
- Credit
- 7,5 ECTS
- Type of assessment
- Written assignment, 5 daysOral examination, 30 minutes with 20 minutes preparation time
- Type of assessment details
- The written exam accounts for one third, is a take-home
assignment on the sampling part. It takes place between the third
and the fourth week of the course.
The oral exam, which accounts for two thirds, takes place after the sample preparation part of the course. 20 min. preparation time before the exam. - Exam registration requirements
Student presentation of curriculum, participation in laboratory exercises and handing in reports are mandatory. Active participation in a minimum of 75% of these activities is required.
- Aid
- All aids allowed
All aids allowed.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
- Re-exam
Same as the ordinary exam.
The two parts of the exam must both be passed individually. If one part is passed at the ordinary exam and the other part is not, reexam will be offered in the part that is not passed at the ordinary exam.
Students who do not fulfil the requirement have to follow the course the following study year.
Criteria for exam assesment
According to the learning outcome.
Course information
- Language
- English
- Course code
- NPLK13003U
- Credit
- 7,5 ECTS
- Level
- Full Degree Master
- Duration
- 1 block
- Placement
- Block 1
- Schedule
- A
- Course capacity
- 30
The number of places might be reduced if you register in the late-registration period (BSc and MSc) or as a credit or single subject student.
Study board
- Study Board of Physics, Chemistry and Nanoscience
Contracting department
- Department of Plant and Environmental Sciences
Contracting faculty
- Faculty of Science
Course Coordinators
- Giorgio Tomasi (gito@plen.ku.dk)
Lecturers
Giorgio Tomasi, Jan H. Christensen