NNEK14004U Fundamentals of beer brewing and wine making

Volume 2015/2016
Education

MSc Programme in Food Science and Technology
MSc Programme in Biology-Biotechnology

Content

Theme 1: Raw material for beer brewing
The taxonomy of the barley genus will be covered with a focus on the phylogenetic structure of the genus. Examples and prospects for (re)introduction of valuable traits in barley cultivars from wild relatives will be discussed. Molecular and biochemical aspects of grain filling, starch accumulation, amylase expression is covered in relation to malting and the development of technical enzymes for malting. Hops, types and cultivars.
Theme 2: The vine and the grapes
The biochemical/physiological basis for canopy management. Grape anatomy, extraction, designing technical enzymes as extraction aids. Analysis of non-volatile components of wine and must.
Theme 3: Pest, disease and infection
Diseases and infections and their vectors. Resistance genes, and how to use these in breeding and engineering.
Theme 4: Flavor and aroma compounds - sensory science
Metabolomics of natural products in must and wine; analysis of volatile compounds, and identification of signature aroma and flavor compounds for different grape varieties. Oxidation, aging and promotion of wine maturation by enzymes.
Theme 5: Natural products: pigments and flavors
Regulation of pigment biosynthesis. Yeast and process derived aroma compounds. Aroma compound assays of predictive value. Technical enzymes as extraction aids. Glycosylation of pigments and aroma compounds. Beta-glucan sequestering of natural products, and the engineering of heat-stable beta-glucanases.
Theme 6: Fermentation
The molecular biology of baker’s yeast. Barley malt and the biochemistry of the fermentation process. Malolactic fermentation. Metabolomics of the fermentation process. PCR-identification of yeast strains and the effect of strains on taste and flavor.

Learning Outcome

The primary outcome will be a sound knowledge of the biochemistry that underlies beer brewing and winemaking and the analytical techniques that support production.

Knowledge
•    Demonstrate an ability to apply cell biology understanding to properties and processes in grape and grain of relevance to wine and beer
Describe biochemical pathways leading to important components of beer and wine
•   Describe biotransformations of compounds during fermentation.
•   Demonstrate overview of spectroscopic and chemical analytical techniques used to guide production.
•   Understand the molecular basis for resistance against pests and disease.

Skills
•    Students with biotechnology background will be able to apply their knowledge for the development of new technical enzymes and ingredients used in beer and winemaking while the students with food science or horticulture background will employ these tools diligently.
•    Apply their knowledge of yeast and malolactic bacteria for strain development, selection and use.
•    Implement existing spectroscopic or analytical methods, or develop new methods for monitoring components, processes and biotransformations in beer and winemaking.
•    Apply their understanding of pest and resistance genes in plant breeding.

Competences
•    Work independently and make intelligent use of scientific literature also from fields outside brewing and winemaking
•    Be theoretically prepared and qualified for applied courses in brewing and winemaking

Primary scientific papers and reviews will accompany all lectures, demonstrations and exercises. These papers define the curriculum and are thus exam relevant. The students are furthermore supplied with a deck of cards with molecules that are relevant to beer brewing and wine making. This "vocabulary" of molecules is also curriculum.

A bachelor degree in biotechnology, food science, horticulture or equivalent education.
Any bachelor degree (biology, chemical engineering, horticulture ....) that endows the student with basic knowledge in biology, chemistry and biochemistry
The core teaching is comprised of lectures delivered by a wide range of speakers, including invited international guest lecturers. These are supplemented by journal clubs and practicals in the form of demonstrations and tastings.
Foreign students who can document competencies equivalent to a Danish bachelor degree are allowed whether they formally have a degree or not.
  • Category
  • Hours
  • Exam
  • 1
  • Lectures
  • 50
  • Practical exercises
  • 9
  • Practical exercises
  • 122
  • Preparation
  • 3
  • Theory exercises
  • 21
  • Total
  • 206
Credit
7,5 ECTS
Type of assessment
Oral examination, 25 minutes
The oral exam is individual. An exam question comprises two main parts: 1) A thematic question typically inspired by one of the lectures; and 2) One of the curriculum papers. Approximately half the time will be spent on discussing the thematic question and the other half discussing the scientific paper typically with a focus on data interpretation or experimental design. Either of the main parts of the exam are suited to also test the student's molecular vocabulary using the "molecule cards".
Exam registration requirements

Participants will do groupwise projects. It is a requirement that the project reports have been submitted and approved.

Aid
Without aids
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Exam period

Exam week at the end of block 1, typically first week of November

Re-exam

If the requirements are not met the reports has to be handed in individually 2 weeks before signing up for re-exam and must be approved.

Criteria for exam assesment

See learning outcome.

The exam gauges the student's ability to discuss selected chemical compounds and biosynthetic pathways in barley, grapevine, hops, yeast and malolactic bacteria of relevance to beer brewing and wine making as well as the associated technologies like enzyme mediated process aids, spectroscopy, genetical engineering, knock-out mutants etc. ... in short, the scientific underpinnings of beer brewing and winemaking