NKEK22000U Advanced Fluorescence Spectroscopy and Microscopy

Volume 2023/2024

MSc Programme in Chemistry
MSc Programme in Nanoscience


The course focus on the theoretical background for and applications of fluorescence spectroscopy and microscopy. The course deals with photophysical properties of molecular systems, in particular related to UV-Vis absorption and fluorescence. Special emphasis is on: energy and electron transfer, polarization (anisotropy), and solvent effects, single molecule spectroscopy and super resolution imaging, time resolved fluorescence and transient absorption. 

Learning Outcome

After having passed the course, the student should be able to:



  Concepts of  the interaction of light with molecules

  Concepts of  photo physical manifestations of the local molecular environment

  Concepts of  for the kinetics involved in deactivation of electronically excited states of molecules

  Concepts of  the basics of photo induced electron transfer

  Concepts of  steady-state and time-resolved anisotropy of fluorescence emission

  Concepts of  the principles of steady-state and time-resolved experimental techniques

  Concepts of   resonance energy transfer and its applications

  Concepts of  application of fluorescence techniques in sensors

 Concepts of  transient absorption 

  Concepts of  single molecule fluorescence techniques

  Concepts of  super resolution fluorescence imaging



- Apply the above mentioned knowledge for understanding and calculating photophysical properties of molecular systems.


- Read contemporary research papers on photophysics, understand them, present them and write an self consistent essay on it.

Joseph R. Lakowicz: "Principles of Fluorescence Spectroscopy", 3rd edition. Lecture notes. Selected research articles.

Photophysic and physical chemistry at a level corresponding to the BSc courses KemiFoto (Photochemistry and Photophysics) or KemiBin (Chemical Bonding), KemiKS (Quantum Chemistry and Theoretical Spectroscopy)

Academic qualifications equivalent to a BSc degree is recommended.
Lectures, student presentations, discussions, and preparation individually and/or in groups.
  • Category
  • Hours
  • Lectures
  • 18
  • Class Instruction
  • 18
  • Preparation
  • 90
  • Project work
  • 40
  • Exam
  • 40
  • Total
  • 206
7,5 ECTS
Type of assessment
Oral examination, 25 min (no preparation time)
Type of assessment details
Oral exam: based on the written essay of the student, the material from the assignment, and the general curriculum of the course.
Exam registration requirements

Submission of written essay before deadline: 5 page essay on specific topic and research papers (assignment). To be submitted 7 days after assignment has been posted on digital exam. 

Without aids
Marking scale
7-point grading scale
Censorship form
No external censorship
Several internal examiners

Same as ordinary exam. A new 5 page essay on specific topic based on curriculum and research papers will be assigned, to be submitted 5 days after assignment.

Criteria for exam assesment

see learning outcomes