NBIK16001U NMR Spectroscopy

Volume 2024/2025

MSc Programme in Biochemistry
MSc Programme in Physics


The aim of the course is for the student to learn basic density operator theory applied to nuclear magnetic resonance (NMR) spectroscopic experiments. This theoretical foundation will be used to analyze how various 1D and multidimensional NMR experiments can be applied for molecular identification and in the study of molecular structure and dynamics. Topics covered will include nuclear spin, chemical shift, scalar and dipolar couplings, relaxation, multi-dimensional spectroscopy, solid-state NMR and the NMR spectrometer. The concepts will be demonstrated by spectra of biological molecules such as proteins and carbohydrates.

Learning Outcome

Having followed this course the student should have acquired the following:


  • About the basic properties of nuclear spin and density operator theory
  • About chemical shift,
  • About multidimensional NMR spectroscopy
  • About basic solid-state NMR
  • About the components of an NMR spectrometer
  • About signal acquisition and processing


  • Be able to process 1D and multidimensional experimental NMR data
  • Be able to analyze NMR spectra for assignment of chemical shifts
  • Be able to analyze the data from T1 and T2 relaxation experiments
  • Be able to calculate the effect of basic NMR pulse sequences
  • Be able to interpret basic NMR spectra in terms of molecular structure
  • Be able to plan an NMR spectroscopic experiment


After the course the student will have competences in critically evaluating and discussing NMR spectroscopic experiments and the results of these. The student will have competences in presenting and discussing NMR spectroscopy in a precise and comprehensive scientific language.

See Absalon.

This course will assume previous knowledge of basic applied NMR spectroscopy corresponding to what is taught in organic chemistry.

Academic qualifications equivalent to a BSc degree is recommended.
Lectures, colloquia and lab excercises. NMR software.
  • Category
  • Hours
  • Lectures
  • 14
  • Class Instruction
  • 28
  • Preparation
  • 142
  • Theory exercises
  • 14
  • Laboratory
  • 4
  • Exam
  • 4
  • Total
  • 206

Continuous oral feedback (both collective and individual).
Written feedback on written assignments.

7,5 ECTS
Type of assessment
On-site written exam, 4 hours under invigilation
Type of assessment details
Exam registration requirements

7 written assignments handed in during the course.


Only certain aids allowed


Marking scale
7-point grading scale
Censorship form
No external censorship
Several internal examiners

The same as ordinary exam.

If less than 10 students are enrolled, the exam will be an oral examination in 25 minutes, with no preparation, no aids.

The 7 written assignments must be handed in and approved by the teacher no later than three weeks before the reexamination. 

Criteria for exam assesment

In order to obtain the grade 12 the student should convingcingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.