SBRI19011U Translational Tools II – New Methods
BRIDGE - Translational Excellence Programme
A two-year postdoctoral fellowship in translational medicine
The main themes covered in the course include:
- Stem cells, induced pluripotent stem cells, and their applications in disease modeling
- Precision nuclease-based genome editing
- Proteolysis targeting chimera (PROTACs)
- Mass spectrometry for understanding drug mechanisms
- High resolution microscopy
On completion of the course, the participants should be able to:
- Explain the rationale for the utilization of several advanced
research methodologies to characterize protein mechanisms of action
in vivo with a focus on three general areas:
- Gene editing
- Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery
- Culture and use mouse embryonic stem cells
- Design of constructs for CRISPR-based gene targeting
- Identifying targets of PROTACs using mass spectrometry
- Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins
- Identify and evaluate advantages and limitations of the use of above listed methods.
- Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
- Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public
Course literature is published on Absalon.
Course literature includes:
The next generation of CRISPR-Cas technologies and applications. Pickar-Oliver A, Gersbach CA. Nat Rev Mol Cell Biol. 2019 Aug;20(8):490-507. PMID: 31147612
PROTACs: An Emerging Therapeutic Modality in Precision Medicine. Dhanusha A. Nalawansha and Craig M. Crews. Cell Chemical Biology. 2020, 27(8):998-1014.
Chemoproteomics and Chemical Probes for Target Discovery. Drewes G1, Knapp S2. Trends Biotechnol. 2018 Dec;36(12):1275-1286. PMID: 30017093
Induced pluripotent stem cells in disease modelling and drug discovery. Rowe RG, Daley GQ. Nat Rev Genet. 2019 Jul;20(7):377-388. PMID: 30737492
Microscopy-Based High-Content Screening. Boutros M, Heigwer F, Laufer C. 2015. Cell 163(6):1314-25. doi: 10.1016/j.cell.2015.11.007.
- Theory exercises
Automatic registration upon appointment in the Translational Excellence Programme
- 0 ECTS
- Type of assessment
- Continuous assessmentCourse participationAttendance and active participation
- Exam registration requirements
Participants are automatically registered for the Examination upon course registration.
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assesment
Active contribution and course participation according to the BRIDGE Guidelines.
- Course code
- 0 ECTS
- Part Time Master
- See course dates and course programme in Absalon
*This course is offered in autumn 2022 for BRIDGE 2021-2023.
- Course capacity
- 15 participants
- Course is also available as continuing and professional education
- Study board
- Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
- Center for Protein Research
- Faculty of Health and Medical Sciences
- Chuna Ram Choudhary (15-6f74817a6d3a6f747b8170746d7e854c6f7c7e3a77813a7077)
- Takeo Narita (12-7d6a746e7837776a7b727d6a496c797b37747e376d74)
- Marie Sofie Yoo Tollenaere-Larsen (12-766a7b726e37756a7b7c6e77496c797b37747e376d74)