SBRI19011U Translational Tools II – New Methods

Volume 2021/2022

BRIDGE - Translational Excellence Programme

A two-year postdoctoral fellowship in translational medicine


The main themes covered in the course include:

  • Stem cells, induced pluripotent stem cells, and their applications in disease modeling
  • Precision nuclease-based genome editing
  • Proteolysis targeting chimera (PROTACs)
  • Mass spectrometry for understanding drug mechanisms
  • High resolution microscopy
  • Cryo-EM
Learning Outcome

On completion of the course, the participants should be able to:



  • Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas: 
    • Gene editing
    • PROTACs
    • Cryo-EM
  • Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery



  • Culture and use mouse embryonic stem cells
  • Design of constructs for CRISPR-based gene targeting
  • Identifying targets of PROTACs using mass spectrometry
  • Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins



  • Identify and evaluate advantages and limitations of the use of above listed methods.
  • Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
  • Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public


Course literature is published on Absalon.


Course literature includes:

The next generation of CRISPR-Cas technologies and applications. Pickar-Oliver A, Gersbach CA. Nat Rev Mol Cell Biol. 2019 Aug;20(8):490-507. PMID: 31147612


PROTACs: An Emerging Therapeutic Modality in Precision Medicine. Dhanusha A. Nalawansha and Craig M. Crews. Cell Chemical Biology. 2020, 27(8):998-1014.


Chemoproteomics and Chemical Probes for Target Discovery. Drewes G1, Knapp S2. Trends Biotechnol. 2018 Dec;36(12):1275-1286. PMID: 30017093


Induced pluripotent stem cells in disease modelling and drug discovery. Rowe RG, Daley GQ. Nat Rev Genet. 2019 Jul;20(7):377-388. PMID: 30737492


Microscopy-Based High-Content Screening. Boutros M, Heigwer F, Laufer C. 2015. Cell 163(6):1314-25. doi: 10.1016/​j.cell.2015.11.007.

Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme
The course will be organized with a mixture of scientific seminars by invited speakers including lectures about stem cells, PROTACs, CRISPR-based genome engineering, high-resolution microscopy, cryo-EM, as well as the applications of modern proteomics technologies for translational research. In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.
  • Category
  • Hours
  • Lectures
  • 10
  • Preparation
  • 5
  • Theory exercises
  • 5
  • Exercises
  • 20
  • Total
  • 40
Continuous feedback during the course of the semester
Type of assessment
Continuous assessment
Course participation
Attendance and active participation
Exam registration requirements

Participants are automatically registered for the Examination upon course registration.

All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assesment

Active contribution and course participation according to the BRIDGE Guidelines.