SBRI19011U Translational Tools II – New Methods
BRIDGE - Translational Excellence Program
The main themes covered in the course include:
- Stem cells, induced pluripotent stem cells, and their applications in disease modeling
- Precision nuclease-based genome editing
- Proteolysis targeting chimera (PROTACs)
- Mass spectrometry for understanding drug mechanisms
- High resolution microscopy
- Cryo-EM
On completion of the course, the participants should be able to:
Knowledge
- Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on two general areas:
1) gene editing
2) cryo-EM
- Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery
Skills
- Culture and use mouse embryonic stem cells
- Mass spectrometry sample preparation for identifying targets of PROTACs
- Analyse the physical principles that govern the use of light microscopy methods for the characterization of proteins in living cells.
- Apply different microscopy methods commonly used for analysis of cellular protein signalling events, including the use of advanced imaging analysis and live cell imaging.
Competences
- Identify and evaluate advantages and limitations of the use of above listed methods.
- Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
- Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public
The next generation of CRISPR-Cas technologies and applications. Pickar-Oliver A, Gersbach CA.Nat Rev Mol Cell Biol. 2019 Aug;20(8):490-507. PMID: 31147612
Chemoproteomics and Chemical Probes for Target Discovery.
Drewes G1, Knapp S2. Trends Biotechnol. 2018 Dec;36(12):1275-1286.
PMID: 30017093
Induced pluripotent stem cells in disease modelling and drug discovery.
Rowe RG, Daley GQ. Nat Rev Genet. 2019 Jul;20(7):377-388. PMID: 30737492
PROteolysis TArgeting Chimeras (PROTACs) - Past, present and future. Pettersson M1, Crews CM2. Drug Discov Today Technol. 2019 Apr;31:15-27. 13. PMID: 31200855
- Category
- Hours
- Course Preparation
- 5
- Exercises
- 20
- Lectures
- 10
- Theory exercises
- 5
- Total
- 40
Automatic registration upon appointment in the Translational Excellence Program
- Credit
- 0 ECTS
- Type of assessment
- Continuous assessmentCourse participationAttendance and active participation
- Exam registration requirements
Participants are automatically registered for the Examination upon course registration.
- Aid
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assesment
Knowledge
- Understand the applications of stem cells and PROTACs in drug discovery
- Understand the principles of gene editing
Skills
- Interpret the experimental data obtained by the above-mentioned methods.
- Present experimental data in an understandable and scientifically sound manner.
- Discuss and critically review articles and research proposal for appropriateness in the utilization of methodological approaches for characterization of protein function in vivo.
- Design a tailored research plan to test specific hypotheses related to the characterization of protein function in mammalian cells using relevant and informative methods
Competences
- Project planning.
- Data acquisition and interpretation.
- Scientific communication both written and oral
- Scientific communication both written and oral
Course information
- Language
- English
- Course code
- SBRI19011U
- Credit
- 0 ECTS
- Level
- Part Time MasterPh.D.
- Duration
- Placement
- Spring
- Schedule
- See course dates and course programme in Absalon
- Course capacity
- 15 participants
- Continuing and further education
- Study board
- Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
Contracting department
- Center for Protein Research
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinators
- Chuna Ram Choudhary (15-6b707d7669366b70777d6c70697a81486b787a36737d366c73)
- Moreno Papetti (14-7476796c7576357768776c7b7b70476a777935727c356b72)
- Takeo Narita (12-7966706a74337366776e79664568757733707a336970)