NPLK13004U Advanced Analytical Chemistry - Chromatography and Mass Spectrometry
MSc Programme in Environmental Science
MSc Programme in Chemistry
Today, chromatography and mass spectometry are by far the most
common used analytical techniques. A thorough understanding of the
chromatographic theory, and operating mechanism of ionisation
techniques and mass analysers is essential for method selection,
development and optimisation. This is an advanced course in
chromatography and mass spectrometry for chemical analysis of
organic compounds. The course is a natural extension of any basic
course in analytical chemistry for everybody who is going to use
modern hyphenated techniques such as GC-MS, LC-MS(MS) for analysis
of complex mixtures.
The course covers chromatographic separation of small molecule organic compounds with special emphasize on the molecular mechanism and theory of analyte-column interactions for gas, supercritical fluid and liquid chromatography, and the theory of ionization, fragmentation, mass-to-charge separation, ion detection and data interpretation for all common mass spectrometers and ionization techniques. The theory is taught using lectures, colloquia sessions, laboratory and theoretical exercises supplemented with guest-lectures of selected topics.
After completing the course the student should have acquired:
Knowledge - the student has:
-A solid theoretical understanding of chromatography and mass spectrometry at an advanced level.
-Som experience in adressing complex analytical problems, and acquaintance with tools to solve them.
- Hands-on experience with advanced and diverse chromatographic systems and mass spectrometers.
Skills - the student will be able to:
-Describe, compare and contrast, operate and optimize common ionization techniques and mass analysers.
-Describe, perform and evaluate exact mass measurements and fragmentation experiments using mass spectrometers.
-Asses, quantify and compensate for matrix effects in ionization processes.
-Use mass spectrometry and fragmentation theory to annotate mass spectra.
-Describe and use the rate theory, the plate theory, the linear solvent strength model and the hydrophobic subtraction model to optimize chromatography, select and characterize columns and to explain experimental data.
-Use chromatographic theory to predict retention behavior.
-Describe, compare and contrast, operate common chromatographic techniques.
-Optimise in a time- and cost effective manner any chromatographic separation.
-Critically assess reported chromatographic and/or mass spectrometric research.
-Select suitable analytical platform for the separation, detection and quantification of analytes from complex matrices including selection and assessment of column, mobile phase and chromatographic parameter; ionisation mode, source and mass analyser parameterisation.
Competences - enables the student to:
- Plan and perform analysis of complex mixtures using modern chromatographic techniques and high-end detectors, including the selection of method, columns and mode of detection, as well as optimization of chromatographic and mass spectrometric conditions for all common instrumental analytical separation methods.
Specific literature is given on Absalon. Previous years we used:
Recent editions of:
"Introduction to Modern Liquid Chromatography", by
Lloyd R. Snyder; Joseph J. Kirkland and John W. Dolan, Wiley
"Mass Spectrometry. Principles and Applications", by Edmond de Hoffmann and Vincent Stroobant, Wiley 2007.
“High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model”, by Lloyd Snyder and John Dolan, Wiley-Blackwell 2007.
“Interpretation of Mass Spectra”, by Fred McLafferty and Frantisek Turecek, University Science Books, 1993.
Furthermore, selected articles will be used.
- 7,5 ECTS
- Type of assessment
- Oral examination, 20 min25 min. preparation
- Exam registration requirements
Student presentation of curriculum, participation in laboratory exercises and handling in reports are mandatory. Active participation in a minimum of 75% of these activities is required.
- All aids allowed
All aids allowed.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
There can not be dispensated for the requirement of laboratory exercises, and students who do not fulfil the requirement has to follow the course the following study year.
Criteria for exam assesment
The student should be able to explain, contrast and compare the operating mechanism of a range of chromatographic techniques, ionisation sources and mass spectrometers, preferably with examples from the laboratory exercises. The student should be able to explain and use the theoretical models for chromatography, which we used during the course.
- Practical exercises
- Theory exercises
- Project work