NBIK22002U Advanced Protein Science 1 - Biophysical Methods

Volume 2022/2023
Education

MSc Programme in Biochemistry
MSc Programme in Physics

Content

The course is an introduction to biophysical techniques used in protein science to measure structural and biophysical properties of proteins. It includes the biophysical methods of optical spectroscopy (circular dichroism, fluorescence,incl single molecule fluorescence, absorbance), hydrogen exchange mass spectrometry, surface plasmon resonance, isothermal and differential scanning calorimetry, NMR spectroscopy, and small angle x-ray scattering.
The focus is on the theoretical background of the methods, the instrumentation, and on the application of these methods in protein science. The course is a mixture of lectures and group discussions of fundamental topics in each of the methodologies, and for most of these, hands on introduction to both the experimental and analytical tools of these methods.
Teaching Tuesdays and Thursday mornings + hands-on exercises, one exercise pr student.

Learning Outcome

To use the knowledge of the principles of the presented biophysical in evaluations of their application to protein science research projects.

Knowledge:

Knowledge on advanced protein biophysics techniques and instrumentation regarding optical spectroscopy (circular dichroism, fluorescence, absorbance), mass spectrometry, surface plasmon resonance, isothermal and differential scanning calorimetry, NMR spectroscopy, and small angle x-ray scattering.

Skills:

The student will be able to design, execute and present experiments in advanced protein biophysics regarding optical spectroscopy (circular dichroism, fluorescence, absorbance), mass spectrometry, surface plasmon resonance, isothermal and differential scanning calorimetry, NMR spectroscopy, and small angle x-ray scattering.

Competences:

Critically evaluate data obtained in advanced protein biophysics regarding optical spectroscopy (circular dichroism, fluorescence, absorbance), hydrogen exchange mass spectrometry, surface plasmon resonance, isothermal and differential scanning calorimetry, NMR spectroscopy, and small angle x-ray scattering.

See Absalon.

Bachelor degree either in biochemistry, biophysics, nanotechnology, chemistry, or molecular biomedicine.

It is necessary that a basic course in protein science has been passed. It is an advantage that the student has passed Protein Science A or C, or the protein research lab.
Lectures, student presentations, group discussions and laboratory and computer exercises, written reports for evaluation.
  • Category
  • Hours
  • Lectures
  • 14
  • Class Instruction
  • 7
  • Preparation
  • 143,5
  • Theory exercises
  • 21
  • Practical exercises
  • 20
  • Exam
  • 0,5
  • Total
  • 206,0
Oral
Individual
Collective

Oral feedback both individually and in groups on oral presentations. Oral and written feedback on the laboratory report and presentation.

Credit
7,5 ECTS
Type of assessment
Oral examination, 20 min. (no preparation)
Type of assessment details
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Exam registration requirements

In order to be admitted to the exam the students must have performed the following at a satisfactory level:

  • Performed a supervised practical project including designing and executing an experiment, using one of the selected methods of the course.
  • Written one report covering the performed experiment. The report should cover: 1) description and critical evaluation of the method; 2) explanation of typical protocols and strategies used; 3) data evaluation; 4) presentation of the results including graphs, analyses, and calculated results; 5) a critical analysis of the results; 6) estimation of experimental errors and an explanation of these.
Aid
Without aids
Marking scale
7-point grading scale
Censorship form
No external censorship
Several internal examiners
Re-exam

The same as the ordinary exam.

If the requirement of a supervised practical project including designing and executing an experiment, using one of the selected methods of the course, has not been fulfilled, the student will need to take the course again.

If the requirement of a written report has not been fulfilled, it can be fulfilled no later than three weeks before the reexamination by agreement with the course responsible.

Criteria for exam assesment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competencies described under "Learning Outcome".