NKEK14015U The Chemistry of Metal Ions in Biological Systems

Volume 2019/2020
Education

MSc Programme in Biology-Biotechnology
MSc Programme in Chemistry
MSc Programme in Biochemistry
MSc Programme in Environmental Science

Content

This course deals with the chemical reactivity and function of metal ions in biological systems. This field of research (also called bioinorganic chemistry or biological inorganic chemistry) is a relatively new interdisciplinary field, which has connections to biochemistry, inorganic chemistry, biology, biophysics and pharmacology. The central purpose is to illustrate how metal ions are essential for life. Their function may range from the simple charge balance and structural role to the complex roles in which they transfer electrons, break bonds, make bonds or control biochemical reactions.

The course content is dynamic and will reflect the current hot topics/new discoveries in bioinorganic chemistry. The course will contain selections of the following subjects:

  • Biological trace elements, and their abundance and bioavailability.
  • The coordination chemistry of selected metal ions
  • Bioinorganic model systems.
  • Biological metal ion transport mechanisms (uptake, storage).
  • Metal ion and metal ion complexes’ interaction with proteins and nucleic acids.
  • Metal ions in diseases and medicine. 
  • Dioxygen transport proteins.
  • The coordination chemistry and mechanism of dioxygen activating iron enzymes such as the heme containing cytochrome P450.
  • Dioxygen activating copper enzymes (multi-copper oxidases) and other enzymes in oxygen metabolism such as superoxide dismutase, peroxidases and catalase.
  • Nitrogenase and biological dinitrogen fixation.
  • The bioinorganic chemistry of selected metal ions e.g. zink (hydrolytic enzymes), molybdenum (sulfite oxidase) and nickel (urease, NiFe hydrogenase).
  • Interaction of metal ions with DNA and RNA.
  • Metalloproteins in biological electron transfer (photosynthesis, respiration) and the physical nature of electron transfer.
  • Bioinorganic chemistry in biotechnology, environmental context and in biocatalysis.
  • Introduction to the theory behind experimental techniques used for studying metal ions in biological systems. Examples of such techniques are mass spectrometry (MS), electron paramagnetic resonance spectroscopy (EPR), circular dichroism (CD), Perturbed angular correlation spectroscopy,  X-ray absorption spectroscopies.
 
Learning Outcome

The objective of this course is to give the students a thorough introduction to the function of metal ions in biological systems and make the students able to analyze the structure and function of metal ion containing biomolecules.

After the end of this course the students should be able to:

Knowledge
Describe the different functions metal ions can have in biological systems

Provide an overview over metalloproteins function in biological systems

Provide an overview of biological selection of important metal ions.

Have theoretical insight into which spectroscopic techniques that are appropriate for investigating a specific metalloprotein.

Discuss why too high and/or too low concentrations of trace elements can be harmful to living organisms

Skills
Be able to find and read primary scientific articles within the field and explain the content.

Communicate clearly written and orally issues of bioinorganic chemistry

Describe the function of metalloenzymes on a molecular basis

Competences
Critically evaluate the data presented in scientific articles.

Suggest the possible function of a particular transition metal ion in a biological molecule when presented with structural information about its coordination environment.

Predict whether an enzyme catalysed reaction is likely to involve metal ions and in the positive case, what metal ions are likely.
 

Literature

A textbook on the topic could be: Bertini, Gray, Stiefel & Valentine Biological Inorganic Chemistry – Structure & Reactivity, University Science Books, USA + notes and articles.  See Absalon for detailed information on the textbook.

Basic courses in chemistry and biochemistry at the respective faculties.
Course in chemistry like: General Chemistry for Life Sciences,
Organic Chemistry for Life Sciences.
Course in biochemistry like: Biochemistry 1 or Chemistry of Macromolecules, Cofactors and Metal Ions in Biological Systems.

Academic qualifications equivalent to a BSc degree is recommended.
Lectures, individual work on a project with the possibility of study groups, presentation and discussion of articles, instrument demonstrations, and theoretical exercises.
The course is an inter-faculty course with teachers from SUND(1 week), SCIENCE(6 week). One week will be reserved for project work. Teaching will be on Frederiksberg and Nørre Campus
  • Category
  • Hours
  • Colloquia
  • 15
  • Exam
  • 1
  • Lectures
  • 30
  • Preparation
  • 100
  • Project work
  • 45
  • Theory exercises
  • 15
  • Total
  • 206
Oral
Individual
Collective
Credit
7,5 ECTS
Type of assessment
Oral examination, 30 min.
The oral examination (with 30 min. preparation) includes a presentation of the written report.
Exam registration requirements

Presentation and critique of an article in journal club sessions. Delivery and acceptance of a written project report (10-15 pages) made throughout the course. The written report should be handed in at least 1 week before the oral exam.

Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
External censorship
Re-exam

Same as ordinary exam. 

After adjustments the written report can be submitted again no later than 2 weeks before the reexam.

Criteria for exam assesment

See Learning Outcome