NBIB10009U Gene Technology (Gentek)

Volume 2019/2020
Education

BSc Programme in Biology
BSc Programme in Biochemistry

MSc Programme in Biology with a minor subject

Content

The theoretical part includes:

  • A thorough review of gene technology methods
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals


The practical part includes:

  • The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
  • Site-directed mutagenesis
  • Real-time PCR for mRNA quantification and SNP analysis
  • Application of reporter genes to study gene regulation and subcellular protein localisation
  • Yeast 2-hybrid analysis
  • Affinity purification of fusion proteins
  • Pull-down experiments
  • Fluorescence microscopy
  • Internet based gene technology tools
  • Danish and EU law on gene technology
Learning Outcome

Knowledge:

The course provides the student with a detailed and comprehensive knowledge on theoretical and practical modern gene technology. The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.

Skills:

The student gains core competences within theoretical and practical modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods and internet based tools. The student obtains experience with critical interpretation and treatment of original molecular biology data, and gets acquainted with work routines in a gene technology laboratory in relation to Danish and EU law on gene technology and genetically modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.
Personal: The student learns to collaborate in groups and prepare a written presentation and discussion of their obtained experimental data.

Competencies:

The competencies obtained by the student include

  • to interpret data from the scientific literature and put them in a molecular biology/gene technology context
  • to design their own experiments
  • to work in a classified laboratory in accordance with legal regulations
  • to work in small teams
  • to give a detailed description of and evaluate the gene technology tools presented during the course
  • to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
  • to describe and explain individual steps in a presented gene technology manual
Literature

See Absalon.

Knowledge corresponding to the content of the courses: Basic Biochemistry, Basic Molecular Biology and Basic Cell Biology.
Lectures, problem solving and laboratory experiments.
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.
  • Category
  • Hours
  • Colloquia
  • 28
  • E-Learning
  • 8
  • Exam
  • 4
  • Lectures
  • 28
  • Practical exercises
  • 90
  • Preparation
  • 254
  • Total
  • 412
Written
Collective
Credit
15 ECTS
Type of assessment
Written examination, 4 hours under invigilation
The course has been selected for ITX exam on Peter Bangs Vej.
Aid
All aids allowed

The University will make computers and power available to students taking written exams with invigilation in the University’s building on Peter Bangs Vej 36 (ITX). Students are therefore not permitted to bring their own computers, tablets or mobile phones. If textbooks and/or notes are permitted, according to the course description, these must be in paper format or on a USB flash drive.

Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Re-exam

The same as the ordinary exam.

Criteria for exam assesment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.