NNEK17000U Laboratory Methods in Nutrition
MSc Programme in Human Nutrition
MSc Programme in Food Innovation and Health
This course is aimed to provide supplementary knowledge reflecting state–of–the-art technologies and methodologies utilized in studies on nutritional and health in academia and industry. The students will learn about:
current laboratory methodologies in nutrition
storage and preparation of biological samples
extraction and separation methods (centrifugation and chromatographic techniques)
analyte detection and quantitation (mass spectrometry, spectrophotometry, immunoassays)
analytical method development, optimization and validation
application of omics techniques in nutritional science with focus on metabolomics
writing validation sheet, protocol and report
oral data presentation
terminology used in description and characterization of analytical methods for nutritional applications
the principles, pros and cons of various state–of–the-art analytical methods
sources of analytical errors
process of analytical method development, optimization and validation
problems occurring during the preparation and analysis of complex biological matrices
limitations of the analytical methods and quantitative results
both theoretical and practical aspects of laboratory work related to nutritional studies
analytical method development and method validation
to use and critically evaluate common laboratory methods in nutritional science
unknown compounds identification
systematic evaluation of analytical results
to evaluate and critically judge analytical results and methodologies in nutritional studies
be able to choose suitable analytical approaches for specific purposes
Course literature will be announced at study start on the course’s Absalon page.
Previous basic knowledge of biochemistry and chemistry with related laboratory activities is recommended.
Practical exercises will consist of theoretical preparations (experiment design and calculations) and laboratory work.
Experiments are performed by the students themselves and in cases of highly advanced analytical methods together with the supervisor as demonstration.
Students will solve and discuss real case problems in the groups and with the supervisor.
Results will be summarized in a scientifically written protocols and reports and a selected topic orally presented during a common mini–conference.
The students are expected to act as opponents for other groups, but the supervisors and the
course responsible will do the final judgment of the reports and oral presentations.
- Class Exercises
- 7,5 ECTS
- Type of assessment
- Oral examination, 20 minEvaluation is based on the protocols, reports from the exercise and final oral examination.
At the examination, a selected topic will be presented from the students as oral presentation, the results from the written assignments will be discussed and general knowledge on theoretical and practical aspects of laboratory work will be tested. The oral examination weighs100 % and lasts for 20 minutes
- Exam registration requirements
Written assignments (protocols, reports from the exercise) must be delivered and approved two weeks prior to oral examination
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
Internal grading. One assessor
If the student did not fulfill the exam requirements regarding approval of written assignments, the student should hand-in the failed report(s) and pass it two weeks before the re-examination. The re-examination will be oral and based on the presentation of a report.
Criteria for exam assesment
Please see ”Learning Outcome”