NBIK13005U Experimental Higher Model Organisms
MSc programme in Biology
MSc Programme in Biochemistry
Theoretical exercises, colloquia and lectures will give a critical review of the use of genetic model systems and strategies used in basic and applied research. Laboratory exercises will include a range of techniques such as
1) Vector construction and genome, transcriptome and/or proteome database mining
2) Genomic manipulations via homologous recombination, RNA interference and random insertional mutagenesis,
3) Stable transformation and transient transfection,
4) Transgenic analyses with marker enzymes and/or tagged proteins.
The techniques will be applied to cells and organisms such as vertebrate cell lines, the worm C. elegans, the fruit fly D. melanogaster, the moss P. patens and/or the flowering plant A. thaliana.
By the end of the course, students are expected to have the
following knowledge, skills and competences:
Knowledge of the research fields with model organisms included in the curriculum covered by assigned reading, lectures, seminar presentations and written reports.
- Explain developments in the fields presented by the teachers
- Describe in detail the methodologies used in these fields
- Explain and exemplify the technologies used in these fields
- Present assigned original research papers
- Submit assigned written papers
- Be able to give a detailed description of and evaluate the exercises presented during the course
- Discuss and criticize research articles orally and in writing
- Interpret different types of experimental data introduced in the course
- Discuss interdisciplinary aspects of the research covered in the course
- Propose experiments to test models and questions raised in research on model organisms.
- Project work
- Theory exercises
- 7,5 ECTS
- Type of assessment
- Continuous assessmentActive participation in all exercises, presentation of published, original research papers, written reports. The grade will be given based on an overall evaluation.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
Re-examination requires an individual, oral presentation by the student to course instructors of:
1- One of the research articles presented by students during the foregoing course
2- One of the bioinformatics problems assigned to students during the foregoing course
3- One of the prosed experiment reports assigned to students during the foregoing course
The oral presentation is set 3 hours (no aids allowed). The preparation time is set for a week (all aids allowed).
Criteria for exam assesment
See Learning outcome.