NSCPHD1001  Comprehensive two-dimensional liquid chromatography (LCxLC)

Volume 2015/2016
Content

 

PLEASE NOTE         

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Ad-hoc Phd-course (study-group) for revision of key litterature on LCxLC. This course will cover:

  • Current status within the field reading introductory reviews (week one)
  • Historic aspects of LCxLC (week two)
  • The importance of first dimension sampling-rate (week three)
  • Practical consideration for fast analysis in the second dimension (week four)
  • Theoretical considerations for implementation of LCxLC (week five)
  • The concept of orthogonality (week six)
Learning Outcome

Skills - the student should be able to:

-Describe the history and current status of LCxLC
-Describe the primary problems and list ways of adressing these problems in order for the LCxLC technique to out-perform current one-dimensional techniques.
-Put the theory of LCxLC into the context of own research project.

Week one:

Jandera, P., Comprehensive two-dimensional liquid chromatograpy – practical impacts  and theoretical considerations. A review. Central European Journal of Chemistry, 2012. 10(3): p. 844-875.

Jandera, P., Programmed elution in comprehensive two-dimensional liquid chromatography. Journal of Chromatography A, 2012. 1255: p. 112-129.

Francois, I., K. Sandra, and P. Sandra, Comprehensive liquid chromatography: Fundamental aspects and practical considerations. A review. Analytica Chimica Acta, 2009. 641(1-2): p. 14-31.

Week two:

Bushey, M.M. and J.W. Jorgenson, Automated instrumentation for comprehensive 2-dimensional high-performance liquid-chromatography of proteins. Analytical Chemistry, 1990. 62(2): p. 161-167.

Davis, J.M. and J.C. Giddings, Statistical-theory of component overlap in multicomponent chromatograms. Analytical Chemistry, 1983. 55(3): p. 418-424.

Week three:

Davis, J.M., D.R. Stoll, and P.W. Carr, Effect of first-dimension undersampling on effective peak capacity in comprehensive two-dimensional separations. Analytical Chemistry, 2008. 80(2): p. 461-473.

Murphy, R.E., M.R. Schure, and J.P. Foley, Effect of sampling rate on resolution in comprehensive two-dimensional liquid chromatography. Analytical Chemistry, 1998. 70(8): p. 1585-1594.

Week four:

Stoll, D.R., et al., Fast, comprehensive two-dimensional liquid chromatography. Journal of Chromatography A, 2007. 1168(1-2): p. 3-43.

Week five:

Guiochon, G., et al., Implementations of two-dimensional liquid chromatography. Journal of Chromatography A, 2008. 1189(1-2): p. 109-168.

Week six:

Gilar, M., et al., Orthogonality of separation in two-dimensional liquid chromatography. Analytical Chemistry, 2005. 77(19): p. 6426-6434.

Rutan, S.C., J.M. Davis, and P.W. Carr, Fractional coverage metrics based on ecological home range for calculation of the effective peak capacity in comprehensive two-dimensional separations. Journal of Chromatography A, 2012. 1255: p. 267-276.

'Analytical Chemistry' and 'Advanced Analytical Chemistry - Chromatography and Mass Spectrometry' or equivalent skills
Credit
3 ECTS
Type of assessment
Oral examination
Written assignment
The student can select examination form of their own choice: either one large or six smaller written hand-ins or one oral presentation on a subject matter related to the course.
Exam registration requirements

Active participation in colloquia session, at least 5 out of 6

Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assesment

Approved written or oral presentation

  • Category
  • Hours
  • Preparation
  • 42
  • Colloquia
  • 18
  • Project work
  • 22,5
  • Total
  • 82,5