NKEK12009U CANCELLED Proteins on a Chip
Biochips are becoming very popular and are now adapted to routine applications in laboratories. In this course, we propose an introduction to the field, which can be of interest to any students willing to measure molecular interactions using commercial biochips or design new biochips. An introduction to standard modes of detection and adapted setups for High-Throughput Screening will also be given.
The course will contain plenary lectures, lab exercises and literature work
There are several assignments:
1- lab reports
2- journal club (oral presentation of an article - 20 mn- and acting as opponent for two other articles presented during the course)
3- literature report about one topic related to the course
PhD students can take a 5 ECTS version of the course corresponding to assignments 1 & 2.
The overall goal of the course is to learn and understand how do the main categories of membrane proteins function. Completing the course the student is expect to have acquired basic principles about:
Interface protein and surface
Protein Immobilization strategies
Surface based techniques for the investigation of proteins
Protein arrays: classical and novel technologies.
Run classical experiments with immobilized proteins.
Discuss scientific articles dealing with use or investigation of proteins
Explain the advantages and inconvenients of protein immobilization
Describe several types of immobilization strategies with their specificities
Describe the principle of several surface based techniques
Based on reviews and articles
25 hours of lecture and exercises / tutorial. ; approx 15 hours of lab exercises + lab reports; literature report
Lab exercises at the Bioanotechnology and Nanomedicine Laboratory (fluorescence microscope, biacore instrument and other biophysical techniques). The students will have to design part of the experimental protocol before the experiments and to write lab reports for each exercise.
Literature work is composed of critical reading of articles during the course (and oral presentation of one of them) and of one report reviewing a topic related to the course.
- Practical exercises
- Project work
- 7,5 ECTS
- Type of assessment
- Continuous assessment- Laboratory exercises are mandatory. Reports of each laboratory exercise should be submitted according to deadlines announced at the beginning of the course.
- Critical reading of articles ( 3 assessments per student)
- Literature report (written) at the end of the course.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
Oral exam. 20 minutes. The requirements for the re-exam are the same as for the ordinary exam. The requirement involving participation in experimental activities cannot be waived and students who do not fulfill this requirement must follow the course during the next academic year.
Criteria for exam assesment
see learning outcomes