NBIB10009U Gene Technology (Gentek)
MSc Programme in Biology
The theoretical part includes:
- A thorough review of gene technology methods
- Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
- Generation of genome- and cDNA libraries
- Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
- Genome-, trancriptome- and proteome analysis
- Production of transgenic animals
- Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
The practical part includes:
- The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
- Site-directed mutagenesis
- Real-time PCR for mRNA quantification and SNP analysis
- Application of reporter genes to study gene regulation and subcellular protein localisation
- Yeast 2-hybrid analysis
- Affinity purification of fusion proteins
- Pull-down experiments
- Fluorescence microscopy
- Internet based gene technology tools
- Danish and EU law on gene technology
Knowledge:
The student gets aquainted with practical laboratory routines
fulfilling the requirements stated by the Danish and EU law on gene
technology.
In the theoritical part the student will obtain detailed knowledge
on
- Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
- Generation of genome- and cDNA libraries
- Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
- Genome-, trancriptome- and proteome analysis
- Production of transgenic animals
- Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
In the experimental part the student will obtain practical knowledge on
- The most important methods to generate recombinant plasmids
- Site-directed mutagenesis
- Real-time PCR for mRNA quantification and SNP analysis
- Application of reporter genes to study gene regulation and subcellular protein localisation
- Yeast 2-hybrid analysis
- Affinity purification of fusion proteins
- Pull-down experiments
- Fluorescence microscopy
- Internet based gene technology tools
- Danish and EU law on gene technology
Skills:
The student gains core competences within theoretical and practical
modern gene technology. The degree of confidence with modern gene
technology/molecular biology obtained during the course allows
him/her to design gene technology/molecular biology experiments
based upon known gene technological methods and internet based
tools. The student obtains experience with critical interpretation
and treatment of original molecular biology data, and gets
acquainted with work routines in a gene technology laboratory in
relation to Danish and EU law on gene technology and genetically
modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory
routines fulfilling the requirements stated by the Danish and EU
law on gene technology.
Personal: The student learns to collaborate in groups and prepare a
written presentation and discussion of their obtained experimental
data.
Competencies:
The competencies obtained by the student include
- to interpret data from the scientific literature and put them in a molecular biology/gene technology context
- to design their own experiments
- to work in a classified laboratory in accordance with legal regulations
- to work in small teams
See Absalon.
- Category
- Hours
- Colloquia
- 28
- E-Learning
- 8
- Exam
- 4
- Lectures
- 28
- Practical exercises
- 90
- Preparation
- 254
- Total
- 412
As
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Continuing Education - click here!
- Credit
- 15 ECTS
- Type of assessment
- Written examination, 4 hours under invigilation---
- Aid
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
Criteria for exam assesment
To obtain the mark 12 the student should
- Be able to give a detailed description of and evaluate the gene technology tools presented during the course
- Be able to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
- Be able to describe and explain individual steps in a presented gene technology manual
- Be able to interpret data from the scientific literature and relate those to the content of the course
Course information
- Language
- English
- Course code
- NBIB10009U
- Credit
- 15 ECTS
- Level
- Bachelor
- Duration
- 1 block
- Placement
- Block 1
- Schedule
- All schedules
- Course capacity
- No limit
- Continuing and further education
- Study board
- Study Board of Biology and Animal Science
Contracting department
- Department of Biology
Course responsibles
- Per Amstrup Pedersen (10-7263726766677475677042646b71306d7730666d)
Lecturers
Rasmus Hartmann-Petersen og Henriette Pilegaard