LBIK10180U Applied Microbiology

Volume 2014/2015
MSc Programme in Agriculture
MSc Programme in Biology-Biotechnology

The aim of the course is to give the students broad theoretical and practical skills in applied microbiology at an advanced level. During the course, the students will obtain hands-on experience with a number of classical as well as new cutting-edge techniques for characterization of microorganisms and for studying interactions between microorganisms. These  include: plate screening, PCR typing, fluorescent in situ hybridization, fluorescent marker gene technology, and quantitative PCR.

The following experimental areas of applied microbiology will be covered:
- Microbial bioprospecting and population analysis: Screening for enzyme or metabolite producers; genotyping of microorganisms and assessment of biodiversity of cultivated populations.
- Detection of specific microorganisms: Application of marker gene tagging and molecular methods to detect and enumerate specific microorganisms in complex environments.
-  Demonstration of activity of specific microorganisms: Methods to demonstrate metabolic activity or specific gene expression by microorganisms in complex environments.

Furthermore, the students will achieve theoretical knowledge on microorganisms related to environmental and biotechnological issues.

The following theoretical areas of applied microbiology will be covered:
- Extremophilic organisms: Strategies for life and metabolic adaptations of cells to extreme conditions. Applications of extremophilic microorganisms.
-  Microbial interactions in agriculture: Plant-microbe and microbe-microbe interactions such as microbiological control of plant pathogens by microorganisms producing antifungal secondary metabolites.
- Aquaculture microbiology: Biology of taste-and-odour producing bacteria in fish ponds and their impact on fish quality.

Learning Outcome

After completing the course the students should be able to:

Classify microorganisms phylogenetically.
Display an overview of extremophilic bacteria and their enzymes as well as the application potential of organisms and enzymes.
Describe methods for investigating interactions between bacteria and fungi.
Disply an overview of methods used for detection of microorganisms and their activity in complex environments.
Display an overview of the influence of microorganisms on the quality of drinking water and aquaculture products.
Reflect over the properties of qualitative and quantitative methods for detection of microorgansims in complex environments.

Analyse the phylogenetic relationships of microorganisms and evaluate their potential for production of enzymes or bioactive molecules.
Evaluate methods for investigation of microorganisms in complex environments and communicate knowledge in this area.

Select and discuss basic and advanced methods for investigation of microorganisms in complex environments.
Evaluate the mutual interactions between selected microorganisms and their potential influence on the environment.
Evaluate the application potential of microorganisms within biotechnology in relation to environmental sciences.

Brock: Biology of Microorganisms, 13th edition. Pearson Education Inc. 2012.
Review papers and original research papers as specified in the course curriculum.
Manual of practical exercises.

The students are expected to have passed a basic course in microbiology.
Teaching at the course will be in the form of practical exercises, lectures, colloquia and work with theoretical, thematic projects.
  • Category
  • Hours
  • Colloquia
  • 20
  • Exam
  • 1
  • Lectures
  • 20
  • Practical exercises
  • 40
  • Preparation
  • 65
  • Project work
  • 60
  • Total
  • 206
7,5 ECTS
Type of assessment
Oral examination, 30 min
A time period of 30 min is used for preparation for the exam.
Weight: Examination in practical reports: 20%. Examination in theoretical reports: 20%. Examination in textbook, reviews and original papers: 60%
Exam registration requirements
75% of the reports from the practical exercises must be approved.
100% of the theoretical theme reports must be approved.
All aids allowed
Marking scale
7-point grading scale
Censorship form
No external censorship
Én intern bedømmer
Criteria for exam assesment

Please refer to the learning outcome.