SHUA11016U Advanced Methods in Protein Analysis
To attain the course attestation, the student shall be able to understand the:
- High throughput methodologies for expression recombinant proteins.
- Common methods used in the characterization of protein-DNA interactions in living cells or tissues including chromatin immunoprecipitation and immunocytochemistry-FISH.
- Reporter-based assays for the analysis of protein function in living cells.
- Principles of mass spectrometry applied to protein analysis and its applications to cell signaling studies, including isotope labeling for MS-based quantitation.
- Sample preparation methods for the analysis of protein posttranslational modifications.
- Interpretation of MS/MS spectra.
- Utilization of light microscopy methods for the characterization of proteins in living cells, including analysis of cellular signaling events using advanced imaging analysis.
- Analysis of protein-protein interactions in living cells using advanced microscopy techniques such as FRAP and FRET.
- 7,5 ECTS
- Type of assessment
- Oral examination, 30 minuteswith 30 minutes preparation time.
- Exam registration requirements
- Course attestation from the course in Advanced Methods for the analysis of protein disease mechanisms: Approved participation in the seminars and training activities, including approved participation in the exercises and article revision.
- All aids allowed
except for external communication devices.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
More than one internal examiner
Criteria for exam assesment
To achieve the maximum grade of 12 the student shall be able to discuss, analyze and evaluate:
- Complete their practical and theoretical exercises in a timely and satisfactory manner.
- Analyze, evaluate and design strategies for the production of recombinant proteins for analytical purposes.
- Demonstrate an understanding of advanced methodologies to study protein-DNA interactions in living cells or tissues
- Demonstrate an understanding of different reporter-based functional assays and their application for functional characterization of proteins in living cells.
- Demonstrate an understanding of the principles of mass spectrometry applied to protein analysis.
- Demonstrate an understanding of the utilization of high-end quantitative proteomics technologies in the characterization of biological systems, with a focus on stable isotope labeling and high-resolution mass spectrometry.
- Demonstrate understanding of light microscopy methods for the functional characterization of proteins in living cells
- Analyze and evaluate the utilization microscopy in for the characterization of protein-protein interactions in living cells.
- Practical exercises