SHUA11016U  Advanced Methods in Protein Analysis

Volume 2013/2014
Education
MSc Programme in Human Biology
Content
This course aims at providing students with an understanding of advanced methods use for the characterization of protein disease related mechanisms. Through introductory lectures by experienced researchers, practical laboratory exercises and student lead seminars, participants will acquire hands on experience with several methodologies aiming at characterizing protein mechanisms of action in living cells. The course will cover methods for high throughput protein expression, analysis of protein-DNA interactions, mass spectrometry based methodologies for quantitative analysis of posttranslational modifications and light microscopy based analysis of protein signaling and protein-protein interactions.
Learning Outcome

To attain the course attestation, the student shall be able to understand the:

  • High throughput methodologies for expression recombinant proteins.
  • Common methods used in the characterization of protein-DNA interactions in living cells or tissues including chromatin immunoprecipitation and immunocytochemistry-FISH.
  • Reporter-based assays for the analysis of protein function in living cells.
  • Principles of mass spectrometry applied to protein analysis and its applications to cell signaling studies, including isotope labeling for MS-based quantitation.
  • Sample preparation methods for the analysis of protein posttranslational modifications.
  • Interpretation of MS/MS spectra.
  • Utilization of light microscopy methods for the characterization of proteins in living cells, including analysis of cellular signaling events using advanced imaging analysis.
  • Analysis of protein-protein interactions in living cells using advanced microscopy techniques such as FRAP and FRET.
A completed Bachelor degree within the Biomedical and Natural Sciences
Seminars, theoretical exercises, laboratory exercises and article revisions
Credit
7,5 ECTS
Type of assessment
Oral examination, 30 minutes
with 30 minutes preparation time.
Exam registration requirements
Course attestation from the course in Advanced Methods for the analysis of protein disease mechanisms: Approved participation in the seminars and training activities, including approved participation in the exercises and article revision.
Aid
All aids allowed

except for external communication devices.

Marking scale
7-point grading scale
Censorship form
No external censorship
More than one internal examiner
Criteria for exam assesment

To achieve the maximum grade of 12 the student shall be able to discuss, analyze and evaluate:

  • Complete their practical and theoretical exercises in a timely and satisfactory manner.
  • Analyze, evaluate and design strategies for the production of recombinant proteins for analytical purposes.
  • Demonstrate an understanding of advanced methodologies to study protein-DNA interactions in living cells or tissues
  • Demonstrate an understanding of different reporter-based functional assays and their application for functional characterization of proteins in living cells.
  • Demonstrate an understanding of the principles of mass spectrometry applied to protein analysis.
  • Demonstrate an understanding of the utilization of high-end quantitative proteomics technologies in the characterization of biological systems, with a focus on stable isotope labeling and high-resolution mass spectrometry.
  • Demonstrate understanding of light microscopy methods for the functional characterization of proteins in living cells
  • Analyze and evaluate the utilization microscopy in for the characterization of protein-protein interactions in living cells. 
  • Category
  • Hours
  • Lectures
  • 35
  • Practical exercises
  • 16
  • Preparation
  • 86
  • Exam
  • 1
  • Total
  • 138