NSCPHD1251 Udtømmende to-dimensionel væskekromatografi

Årgang 2013/2014
Engelsk titel

Comprehensive two-dimensional liquid chromatography

Kursusindhold

Ad-hoc Phd-course (study-group) for revision of key litterature on LCxLC. This course will cover:

  • Current status within the field reading introdutory reviews (week one)
  • Historic aspects of LCxLC (week two)
  • The importance of first dimension sampling-rate (week three)
  • Practical consideration for fast analysis in the second dimension (week four)
  • Theoretical considerations for implementation of LCxLC (week five)
  • The concept of orthogonality (week six)
Undervisningsmateriale

Week one:

Jandera, P., Comprehensive two-dimensional liquid chromatograpy – practicalimpacts  and theoretical considerations. A review. Central European Journal of Chemistry, 2012. 10(3): 844-875.

Jandera, P., Programmed elution in comprehensive two-dimensional liquid chromatography. Journal of Chromatography A, 2012. 1255: p. 112-129.

Francois, I., K. Sandra, and P. Sandra, Comprehensive liquid chromatography: Fundamental aspects and practical considerations-A review. Analytica Chimica Acta, 2009. 641(1-2): p. 14-31.

Week two:

Bushey, M.M. and J.W. Jorgenson, AUTOMATED INSTRUMENTATION FOR COMPREHENSIVE 2-DIMENSIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS. Analytical Chemistry, 1990. 62(2): p. 161-167.

Davis, J.M. and J.C. Giddings, STATISTICAL-THEORY OF COMPONENT OVERLAP IN MULTICOMPONENT CHROMATOGRAMS. Analytical Chemistry, 1983. 55(3): p. 418-424.

Week three:

Davis, J.M., D.R. Stoll, and P.W. Carr, Effect of first-dimension undersampling on effective peak capacity in comprehensive two-dimensional separations. Analytical Chemistry, 2008. 80(2): p. 461-473.

Murphy, R.E., M.R. Schure, and J.P. Foley, Effect of sampling rate on resolution in comprehensive two-dimensional liquid chromatography. Analytical Chemistry, 1998. 70(8): p. 1585-1594.

Week four:

Stoll, D.R., et al., Fast, comprehensive two-dimensional liquid chromatography. Journal of Chromatography A, 2007. 1168(1-2): p. 3-43.

Week five:

Guiochon, G., et al., Implementations of two-dimensional liquid chromatography. Journal of Chromatography A, 2008. 1189(1-2): p. 109-168.

Week six:

Gilar, M., et al., Orthogonality of separation in two-dimensional liquid chromatography. Analytical Chemistry, 2005. 77(19): p. 6426-6434.

Rutan, S.C., J.M. Davis, and P.W. Carr, Fractional coverage metrics based on ecological home range for calculation of the effective peak capacity in comprehensive two-dimensional separations. Journal of Chromatography A, 2012. 1255: p. 267-276.

Extensive knowledge of analytical chemistry, and liquid chromatography in particular
Colloquia sessions based on discussion, and extensive reading on your own. Project work to prepare written or oral exam.
Please contact Nikoline if you should be interested in another round of this course (njn@plen.ku.dk)
  • Kategori
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  • Forberedelse
  • 42
  • Kollokvier
  • 18
  • Projektarbejde
  • 22,5
  • I alt
  • 82,5
Point
3 ECTS
Prøveform
Mundtlig prøve
Skriftlig aflevering
Pass/non-pass. Oral examination or assessment of written report, whatever is more relevant to the student. Internal censorship.
Krav til indstilling til eksamen
Active participation in discussions; well prepared for colloquia sessions.
Bedømmelsesform
bestået/ikke bestået
Censurform
Ingen ekstern censur