NSCPHD1208 Advanced Live Cell Imaging

This summer course for experienced microscopists comprises theory and hands-on time with the most advanced techniques in microscopy today, such as fluorescence lifetime imaging, super resolution, high throughput imaging, live cell imaging, and spectral imaging.

The course is highly relevant to PhD students at SCIENCE. Many powerful microscopy approaches have been developed recently that have the potential to solve important questions in cell biology. In the course, the participants will benefit from the state-of-the-art instrumentation that is part of the newly established Center for Advanced Bioimaging Denmark. In addition, they will learn the theory behind the methods and how to actually utilize their full potential from internationally outstanding experts that will be part of the course. It will be possible for the students to bring their own specimens.

Part of the summer course is the 4th Bioimaging Workshop Copenhagen, where participants are required to contribute with either a poster or an oral presentation. Confirmed guest speakers at the Workshop will be Prof. Alberto Diaspro (University of Genua), Prof Stefan Jakobs (Max Planck Institute for Biophysical Chemistry) and Prof. Jesper Glückstad (Danish Technical University). Participants are furthermore required to give short presentations of their research on the first day of the course and, after the course, to write a report that includes theory and results from one of the microscopic techniques.

Preliminary programme:

Monday, June 24

Morning: Introduction (Alexander Schulz)

• Theory 1: Microscopy basics, theory of fluorescence (Alexander Schulz)
• 4x 10min participant presentations
• Theory 2: Molecular biology and microscopy, Visualization of protein-protein interaction with Bimolecular fluorescence complementation (Michael Lisby)

Afternoon: 4x 10min participant presentations

• Theory 3: Cell physiology, live-cell imaging (Ivana Novak)
• 4x 10min participant presentations
• Theory 4: Introduction to advanced optical instruments, superresolution and spinning-disc microscopy (Michael Hansen)
• 4x 10min student presentations

Evening: Reception

Tuesday, June 25

Morning: Guest lecture by Frauke Alves: Advanced imaging with confocal and two-photon microscopes on live specimen

• Hands-on: confocal and two-photon microscopy (Frauke Alves, Ivana Novak, Nynne Christensen, Michael Hansen, Alexander Schulz)

Afternoon: Guest lecture by Claus Storgaard: High content screening, data management

• Hands-on: High content screening of living yeast cells (Claus Storgaard, Michael Lisby, Nynne Christensen)

Wednesday, June 26

Morning: Guest lecture by Theordorus Gadella: Visualizing protein-protein interaction with Fluorescence lifetime imaging

• Hands-on: Protein-protein interaction, Fluorescence lifetime imaging (Theodorus Gadella, Alexander Schulz, Nynne Christensen, Michael Hansen)

Afternoon: Alexander Schulz: Superresolution microscopy

• Hands-on: Superresolution microscopy (Alexander Schulz, Michael Hansen)

Thursday, June 27

Morning: Theory 5: Image analysis and data handling (Alexander Schulz, Michael Lisby)

• Hands-on: Image analysis (everybody)

Afternoon: Bioimaging Workshop

Evening: Bioimaging Workshop dinner

Friday, June 28

Morning: Bioimaging Workshop

Afternoon: Discussion and course conclusion