NPLK13004U  Advanced Analytical Chemistry - Chromatography and Mass Spectrometry

Volume 2013/2014

Today, chromatography and mass spectometry is by far the most common used analytical techniques. A thorough understanding of the chromatographic theory, and operating mechanism of ionisation techniques and mass analysers is essential for method selection, development and optimisation.

The course covers chromatographic separation of organic compounds with special emphasize on the molecular mechanism and theory of analyte-column interactions for gas and liquid chromatography, and the theory of ionization, fragmentation, mass separation, detection and data-analysis for all kind of mass spectrometers and ionization techniques in common. All common analytical instruments and techniques from GC-FID/MS to UPLC-UV/FLU/MSMS are addressed. The theory is supplemented with computer simulations and expert guest-lectures of selected topics.

Learning Outcome

After completing the course the student should have acquired:

Knowledge - the student has:
-A solid theoretical understanding of chromatography and mass spectrometry at an advanced level.
-Experience in adressing complex analytical problems, and acquaintance with tools to solve them.

Skills - The student will be able to:
-Select suitable analytical platform for the separation, detection and quantification of analytes from complex matrices including selection and assessment of column, mobile phase and chromatographic parameter; ionisation mode, source and mass analyser parameterisation.
-Do exact mass measurements and fragmentation experiments.
-Troubleshoot advanced chromatographic and mass spectrometric equipment.
-Evaluate the validity of an analytical method and its application domain.
-Critically assess reported analytical chemistry research.

Competences - The student can be considered a:
-Matured analytical chemist with the ability to sketch, develop, implement, maintain and evaluate any chromatographic mass spectrometric method for analysis of complex mixtures.

Selected articles will be used. The textbooks will be announced on Absalon, for inspiration consult:

"Introduction to Modern Liquid Chromatography", by Lloyd R. Snyder; Joseph J. Kirkland and John W. Dolan, 3rd Ed., Wiley 2009.

"Mass Spectrometry. Principles and Applications", by Edmond de Hoffmann and Vincent Stroobant, 3rd Ed., Wiley 2007.

The course LKEF10076 Analytical Chemistry or equivalent is a requirement, since all basic theory of separation and mass spectrometry is assumed to be known.
Lectures, student presentations of curriculum, expert lectures, excursions, hands-on experiments, theoretical exercises, computer simulations.
7,5 ECTS
Type of assessment
Oral examination, 30 min
Exam registration requirements
Student presentation of curriculum, participation in laboratory exercises and handling in reports are mandatory. Active participation in a minimum of 75% of these activities is required.
Only certain aids allowed
All written aids and any calculator which does not allow communication
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assesment
See learning outcome.
  • Category
  • Hours
  • Preparation
  • 123
  • Lectures
  • 16
  • Practical exercises
  • 11
  • Theory exercises
  • 10
  • Colloquia
  • 12
  • Excursions
  • 8
  • Exam
  • 10
  • Project work
  • 16
  • Total
  • 206