NBIK13017U Molecular Biotechnology

Volume 2013/2014
Content

The theoretical part includes:


  • A thorough review of organisms used for recombinant protein production
  • Protein purification related to recombinant protein production.
  • Quality control of recombinant proteins
  • Protein quality in relation to application
  • Optimization of recombinant protein production
  • Large scale growth of host organism
  • Industrial recombinant protein production with experts from Industry
Learning Outcome
Knowledge:

Molecular Biotechnology focuses on how to apply a thorough knowledge on fundamental molecular biology, genetics and protein chemistry to rationalize recombinant protein production on a lab scale as well as on an industrial scale. The student will gain in depth knowledge on the organisms used in industry for recombinant protein production. This will include bacteria, eukaryotic microorganisms and mammalian cell lines as well as intact organisms. The student will obtain knowledge to select the most optimal host cell for production of a specific protein.


Skills:
Through a broad coverage of recombinant protein production the student will be able to evaluate benefits and draw backs related to selection of host organism. The student will get familiar with how to optimize recombinant protein production by applying methods from molecular biology, genetics and protein science. The student will be able to critically evaluate the potential of a specific host organism in relation to the intended application of the recombinant protein.

Competencies:
For more complicated proteins recombinant production in large amounts and of premium quality is not straightforward but very complex. Based on the students knowledge on fundamental molecular biology, genetics and protein chemistry the students will learn to approach this complex situation on a rational basis.
Literature
See Absalon
Knowledge corresponding to the content of the courses: Biochemistry 2, Biochemistry 3, Genetechnology, Genetechnology-nano, Protein Science or equivalent.
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.
Lectures, colloquia
  • Category
  • Hours
  • Colloquia
  • 28
  • Lectures
  • 28
  • Preparation
  • 150
  • Total
  • 206
Credit
7,5 ECTS
Type of assessment
Course participation
Attendance and active participation in at least 80% of the colloquia and oral presentation of articles are compulsory
Marking scale
passed/not passed
Censorship form
No external censorship
Re-exam
Written take home assignment - one week
Criteria for exam assesment

To obtain the mark "passed" the student should

  • Be able to give a detailed description of and evaluate the host organisms presented during the course for recombinant protein production.
  • Be able to thoroughly evaluate experimental data from the scientific literature
  • Be able to thoroughly present data from the scientific literature on recombinant protein production