NBIK11009U Experimental Cell Biology

Volume 2013/2014
Education
MSc Programme in Biology
Content
The exercises are divided into different cell biology topics and include a diversity of techniques. For example: growth and transfection of mammalian tissue cell cultures, differentiation and protein interaction assays, tracer techniques to estimate unidirectional transport of organic compunds, fluorescence associated cell sorting, western blots with using different antibodies, quantitave and qualitative cell death assays and bacterial infections. The different techniques will be applied to several types of cells and model organisms.
Learning Outcome
The student will obtain a broad understanding of key methods in practical cell biology through laboratory exercises, reading of research articles, discussions and presentations.


Knowledge:
The student will obtain knowledge about growth and transfection of mammalian tissue cell cultures, differentiation and protein interaction assays, tracer techniques to estimate unidirectional transport of organic compounds, fluorescence associated cell sorting, western blots using different antibodies, quantitave and qualitative cell death assays and bacterial infections.


Skills:
The student will be able to address and answer new questions in cell biology with a variety of techniques. In addition, the students will be trained in presenting their own work and research papers in different ways.


Competencies:
The student will get practical competences in laboratory work and in reading primary research papers. These competences will be obtained through laboratory exercises, reading of research articles, discussions and presentations.
Literature
See Absalon.
The courses in Cell Biology (15 ECTS) and Cellular and Integrative Physiology (15 ECTS) or equivalent courses.
Introduction lessons in theoretical backgrounds and practical classes including summary and discussion of reports.
The course is part of the qualification profile Cell Biology and Physiology. For all courses of the qualification profiles, see Course Portal for Department of Biology.
  • Category
  • Hours
  • Colloquia
  • 20
  • Lectures
  • 20
  • Practical exercises
  • 100
  • Preparation
  • 232
  • Theory exercises
  • 40
  • Total
  • 412
Credit
15 ECTS
Type of assessment
Course participation
Continuous assessment
Activity based attendance in all exercises is mandatory. Presentation of results and scientific articles.
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assesment
To obtain the mark "Passed" the student should:
  • be able to give a detailed description of and evaluate the exercises presented during the course
  • be able to apply the presented tools to design experiments with the potential to answer defined cell biology questions
  • be able to interpret data from the scientific literature and relate those to the content of the course.