NBIB10010U Gene Technology - Nano (GentekN)

Volume 2013/2014
Education
Bachelor Programme in Nanoscience
Content
The course includes:
  • A thourough review of gene technology methods
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and Saccharomyces cerevisiae
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
Learning Outcome
Knowledge:
The student will obtain detailed knowledge on
  • gene technology methods
  • cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and Saccharomyces cerevisiae
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals

Skills:
The student gains core competences within modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods. The student obtains experience with critical interpretation and treatment of original molecular biology data.

Competencies:
The two most important competences obtained by the student are to comprehend scientific data and put them in a genetechnology/molecular biology context and to use the genetechnology toolbox to solve real genetechnological/​molecular biology problems.
Literature
See Absalon.
Lectures, problem solving.
The course is offered to students in Nanotechnology and related educations. Knowledge corresponding to the content of the course: Nanobio1.
  • Category
  • Hours
  • Colloquia
  • 28
  • Exam
  • 4
  • Lectures
  • 28
  • Preparation
  • 146
  • Total
  • 206
Credit
7,5 ECTS
Type of assessment
Written examination, 4 hours under invigilation
---
Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assesment
To obtain the mark 12 the student should
  • Be able to give a detailed description of and evaluate the gene technology tools presented during the course
  • Be able to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
  • Be able to describe and explain individual steps in a presented gene technology manual
  • Be able to interpret data from the scientific literature and relate those to the content of the course