NBIB10009U Gene Technology (Gentek)

Volume 2013/2014
Education
Bacheloruddannelsen i biologi
Content
The theoretical part includes:
  • A thorough review of gene technology methods
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals

The practical part includes:
  • The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
  • Site-directed mutagenesis
  • Real-time PCR for mRNA quantification and SNP analysis
  • Application of reporter genes to study gene regulation and subcellular protein localisation
  • Yeast 2-hybrid analysis
  • Affinity purification of fusion proteins
  • Pull-down experiments
  • Fluorescence microscopy
  • Internet based gene technology tools
  • Danish and EU law on gene technology
Learning Outcome
Knowledge:
The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.

In the theoritical part the student will obtain detailed knowledge on
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
In the experimental part the student will obtain practical knowledge on
  • The most important methods to generate recombinant plasmids
  • Site-directed mutagenesis
  • Real-time PCR for mRNA quantification and SNP analysis
  • Application of reporter genes to study gene regulation and subcellular protein localisation
  • Yeast 2-hybrid analysis
  • Affinity purification of fusion proteins
  • Pull-down experiments
  • Fluorescence microscopy
  • Internet based gene technology tools
  • Danish and EU law on gene technology

Skills:
The student gains core competences within theoretical and practical modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods and internet based tools. The student obtains experience with critical interpretation and treatment of original molecular biology data, and gets acquainted with work routines in a gene technology laboratory in relation to Danish and EU law on gene technology and genetically modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.
Personal: The student learns to collaborate in groups and prepare a written presentation and discussion of their obtained experimental data.


Competencies:
The competencies obtained by the student include
  • to interpret data from the scientific literature and put them in a molecular biology/gene technology context
  • to design their own experiments
  • to work in a classified laboratory in accordance with legal regulations
  • to work in small teams
Literature
See Absalon.
Knowledge corresponding to the content of the courses: Basic Biochemistry, Basic Molecular Biology and Basic Cell Biology.
Lectures, problem solving and laboratory experiments.
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.
  • Category
  • Hours
  • Colloquia
  • 28
  • E-Learning
  • 8
  • Exam
  • 4
  • Lectures
  • 28
  • Practical exercises
  • 90
  • Preparation
  • 254
  • Total
  • 412
Credit
15 ECTS
Type of assessment
Written examination, 4 hours under invigilation
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Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assesment
To obtain the mark 12 the student should
  • Be able to give a detailed description of and evaluate the gene technology tools presented during the course
  • Be able to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
  • Be able to describe and explain individual steps in a presented gene technology manual
  • Be able to interpret data from the scientific literature and relate those to the content of the course