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NPLB15008U Thematic Course:
Experimental Molecular Biology I
The exercises are typically scheduled in the afternoons from
1pm to ca. 5pm and are complemented by lectures and journal clubs
in the mornings (3-4 days per week).
Continuing and further
Study Board for the Biological Area
Department of Plant and Environmental Sciences
Department of Food and Resource Economics
Department of Veterinary Disease Biology
Meike Burow (3-6f647742726e6770306d7730666d)
Yumiko Sakuragi (3-7b756342726e6770306d7730666d)
Saved on the
BSc Programme in Biology-Biotechology
The course offers experimental platforms to acquire new skills
with regard to methods in molecular biology and biotechnology. The
choice of topics ensures that the students learn standard
techniques relevant to molecular biology, bioinformatics,
biochemistry and physiology. Additionally, the course comprises an
introduction to basic concepts of ethics and philosophy of
The students will gain experiences in a wide-range of both basic
and advanced experimental methods in molecular biology, e.g.
extraction of DNA and RNA, PCR, RT-PCR, real-time PCR, in-situ
hybridization, cloning, primer and vector design, transformation of
prokaryotic and eukaryotic organisms, cultivations of cell
cultures, heterologous gene expression, protein purification,
immuno blotting, histochemical analyses, enzyme kinetics, flow cell
cytometry, bioimaging and bioinformatics. A wide-range of
experimental organisms is employed including bacteria, yeast,
fungi, plants and mammalian cells, with an emphasis that the
methods and basic scientific principles taught have general
The course mainly aims at providing both practical experience
and theoretical knowledge of basic principles and methods in
experimental molecular biology research in combination with an
introduction to general concepts of ethics and philosophy of
science within the field.
After completing the course the students should be able to:
Describe basic principles and analytical methods used in
molecular biology research.
Explain how these principles and methods can be exploited in
practical experiments aimed at reaching distinct research
Describe basic theoretical aspects of scientific research
Describe selected central principles of ethics philosophy of
Carry out experiments using a number of general methods in
molecular biology, biochemistry and cell biology.
Analyse, interpret and conclude from results acquired in the
Summarise and present scientific concepts and own research
results to fellow scientists through written and oral
Interpret and discuss research in the context of theoretical
and ethical principles.
Use e.g IT tools in creative processes in the context of
project management and presentation.
Work independently as well as together with other students to
plan carry out defined experimental work.
Reflect upon the empiric basis of research within the field of
biotechnology and understand and contribute to broader ethical and
societal discussions on the use of
Laboratory manuals including theoretical background for each
practical exercise is made available on Absalon together with the
research articles discussed in the journal clubs and a compendium
for ethics and biotechnology. The literature changes from year to
year dependent on the chosen topics.
See examples on course literature:
1) "The good, the right and the fair. An introduction to
ethics", Mickey Gjerris et al. College publication.
2) “Philosophy of Science: A Very Short Introduction”, Samir
Okasha. Oxford University Press – Publisher.
The following book is optional:
“Principles and Techniques of Biochemistry and Molecular Biology
Paperback”, Keith Wilson (Editor) and John Walker (Editor).
Note that the text books can change until one week before the
Teaching and learning methods
The course comprises lab exercises with written
lab reports, lectures and journal clubs. The students carry out
different experiments as specified in the description of the
exercises (typically in the afternoon from 1pm to ca. 5pm although
this may vary). The results of the exercises are discussed in
follow-up sessions. The exercises are complemented by lectures and
journal clubs in the mornings (3-4 days per week) which provide the
theoretical background for the experiments.
Participation in courses which provide basic
knowledge in biochemistry and molecular genetics is
Exam topics include theories and methods taught in practical
exercises, journal club articles and philosophy of science
Exam registration requirements
5 out of 7 reports must be accepted in order to be eligible for
All aids allowed
NB: If the exam is held at the ITX, the ITX will provide
computers. Private computer, tablet or mobile phone CANNOT be
brought along to the exam. Books and notes should be brought on
paper or saved on a USB key.
7-point grading scale
Reexamination is an oral examination, 30 min. No preparation
If the exam registration requirement is not met, the student has
to follow the course the following year as the laboratory exercises
need to be followed.
Criteria for exam assesment
In order to obtain the grade 12 the student should convincingly
and accurately demonstrate the knowledge, skills and competences
described under Learning