NBIK13017U Molecular Biotechnology
MSc Programme in Biology
MSc Programme in Biochemistry
MSc Programme in Biology with a minor subject
The theoretical part includes:
- A thorough review of organisms used for recombinant protein production
- Protein purification related to recombinant protein production.
- Quality control of recombinant proteins
- Protein quality in relation to application
- Optimization of recombinant protein production
- Large scale growth of host organism
- Industrial recombinant protein production with experts from Industry
Molecular Biotechnology focuses on how to apply a thorough
knowledge on fundamental molecular biology, genetics and protein
chemistry to rationalize recombinant protein production on a lab
scale as well as on an industrial scale. The student will gain in
depth knowledge on the organisms used in industry for recombinant
protein production. This will include bacteria, eukaryotic
microorganisms and mammalian cell lines as well as intact
organisms. The student will obtain knowledge to select the most
optimal host cell for production of a specific protein.
The student gains core competences within recombinant protein
production as an important tool in the research laboratory and a
billion dollar business for the biotech industry. A number of
different cell factories are available for recombinant protein
production. The degree of confidence with recombinant protein
production obtained during the course allows the student to
evaluate benefits and draw backs related to selection of host
organism and protein purification. The student will get familiar
with how to optimize recombinant protein production by applying
methods from molecular biology, genetics and protein science. The
student will be able to critically evaluate the potential of a
specific host organism in relation to the intended application of
the recombinant protein.
The competences obtained by the student include
to interpret and evaluate data from the scientific literature related to recombinant protein production
to design recombinant protein production and purification experiments
to select an expression host compatible with desired quality of the recombinant protein product
to evaluate possibilities for setting up recombinant protein production on a laboratory and industrial scale
to make a literature study on a subject related to recombinant protein production and make an oral presentation of the outcome.
- 7,5 ECTS
- Type of assessment
- Written assignment, 7 daysWritten take-home assignment.
- Exam registration requirements
The student must make an oral presentation in class. The presentation is 30 minutes long and can be made in groups of two.
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
If the student has not fulfilled the exam registration requirements the student must hand in a written assignment to the teacher no later that two weeks before the reexam. The teacher decides the topic of the assignment and the assingment must be approved.
Criteria for exam assesment
In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.