NBIK13005U Experimental Higher Model Organisms
MSc Programme in Biology
MSc Programme in Biochemistry
MSc Programme in Biology with a minor subject
Theoretical exercises, colloquia and lectures will give a critical review of the use of genetic model systems and strategies used in basic and applied research. Laboratory exercises will include a range of techniques such as
1) Vector construction and genome, transcriptome and/or proteome database mining
2) Genomic manipulations via homologous recombination, RNA interference and random insertional mutagenesis,
3) Stable transformation and transient transfection,
4) Transgenic analyses with marker enzymes and/or tagged proteins.
The techniques will be applied to cells and organisms such as vertebrate cell lines, zebrafish D. rerio, worm C. elegans, fruit fly D. melanogaster, moss P. patens and/or flowering plant A. thaliana.
By the end of the course, students are expected to have the
following knowledge, skills and competences:
Knowledge of the research fields with model organisms included in the curriculum covered by assigned reading, lectures, seminar presentations and written reports.
- Explain developments in the fields presented by the teachers
- Describe in detail the methodologies used in these fields
- Explain and exemplify the technologies used in these fields
- Present assigned original research papers
- Submit assigned written papers
- Be able to give a detailed description of and evaluate the exercises presented during the course
- Discuss and criticize research articles orally and in writing
- Interpret different types of experimental data introduced in the course
- Discuss interdisciplinary aspects of the research covered in the course
- Propose experiments to test models and questions raised in research on model organisms.
- 7,5 ECTS
- Type of assessment
- Continuous assessmentActive participation in all exercises, 1-2 oral presentations of published original research papers, 6-8 written reports. The grade will be given based on an overall evaluation.
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
Several internal examiners.
Re-examination requires an individual, oral presentation of:
1) One of the research articles presented by students during the foregoing course
2) One of the bioinformatics problems assigned to students during the foregoing course
3) One of the prosed experiment reports assigned to students during the foregoing course
The oral presentation is 1 hour (no aids allowed). The preparation time is set for a week (all aids allowed).
Criteria for exam assesment
See Learning outcome.
- Theory exercises
- Project work