NBIA04015U  Plant Molecular Biology (PlantMolBiol)

Volume 2017/2018
Education

Bacheloruddannelsen i biologi

Content

Lectures and seminars cover:

  • reproductive and vegetative development
  • genome analysis and gene regulation
  • light and hormone signaling
  • environmental stress & disease
  • applied plant biotechnology


Laboratory exercises cover:

  • plant development, anatomy and mutants
  • transgenics
  • gene cloning and mapping
  • DNA and protein bioinformatics and model organism genome databasing
Learning Outcome

By attending this course the student will achieve:

Knowledge:
By the end of the course, students are expected to have knowledge of the aspects of plant molecular biology that are included in the curriculum
covered by assigned reading, lectures, seminar presentations and laboratory exercises (see Absalon). Thus, students will be expected to know, among other things, about:

  • reproductive and vegetative development
  • genome analysis and gene regulation
  • light and hormone signaling
  • environmental stress & disease
  • applied plant biotechnology
  • transgenics
  • gene cloning and mapping
  • DNA and protein bioinformatics


Skills:
Students are expected to develop the following skills:

  • Explain the development and anatomy of the model plant Arabidopsis
  • Explain the uses of other model plants and crops
  • Explain how plants assimilate and transport nutrients
  • Explain the occurrence, effects and agricultural uses of the plant hormones (auxin, cytokinin, brassinosteroid, and jasmonic, salicylic, gibberellic and abscisic acids)
  • Explain the principles/steps required for cloning, PCR, sequencing, RT-PCR, mutagenesis, transposon and epitope tagging, reporter and marker genes, plant transformation, epistatic analysis
  • Explain the uses of mutants and how mutant alleles may be cloned
  • Explain forward and reverse genetic screens
  • Explain aspects of plant pathogen interactions including disease resistance and susceptibilities


Competences:
Students are expected to develop competences to:

  • Discuss the advantages and disadvantages of genetically modified plants
  • Discuss and criticize research articles orally and in writing
  • Propose experiments to test scientific models or questions
  • Interpret different types of experimental data introduced in the course
  • Use bioinformatics to search a genome database, annotate the structure of a gene, find mutations in it, identify encoded proteins, compare protein sequences, and propose gene/protein functions

See Absalon.

Students who, by the start of the course, have passed all first year courses and half of the second year courses (corresponding to a total of 90 ECTS-points) of their curriculum, should be well prepared to master the course material.
Lectures, laboratory and computer practicals, group discussions and student seminars of newer articles in high impact journals.
Students must wear lab coats for the exercises.
Written
Oral
Individual
Collective
Continuous feedback during the course of the semester
Credit
7,5 ECTS
Type of assessment
Continuous assessment
Three written, open book exams must be taken as scheduled during the course. In addition, a bioinformatics question set must be submitted prior to the last written exam.

The final grade is determined by the grades of the three open book exams (90%) and the bioinformatics question set (10%).
Students can pass the exam without passing all the part-examinations, if the total grade is passed.
Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Re-exam

The re-exam is a 4 hours written exam with all aids allowed.

 

Criteria for exam assesment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.

  • Category
  • Hours
  • Exam
  • 10
  • E-Learning
  • 16
  • Preparation
  • 66
  • Lectures
  • 16
  • Colloquia
  • 8
  • Practical exercises
  • 64
  • Theory exercises
  • 6
  • Project work
  • 20
  • Total
  • 206