NPLB13001U Plant Genomics
BSc Programme in Biology-Biotechnology
BSc Programme in Biology
This intense course is an advanced course in theoretical and applied genomics with a focus on, but not limited to plant technology and examples.
The Big Picture: Introduction and Overview
Crash course, Bioinformatics* and Crash course, Molecular genetics
Genomes: Model plants versus crops
Next-generation sequencing technologies*
Gene expression
Transcription factors, focus on MYB family
From microarrays to next-generation sequencing (RNA-seq)
Forward and reverse genetics
Genetic markers and Quantitative Trait Loci
Genotype – Phenotype; Map based cloning and QTL dissection
Two very different plant genomics models: rice and moss
Functional Genomics
Metabolomics
Biomarkers and RNAi/siRNA*
Student workshop on four functional genomics assignments
Functional genomics debate on the findings of the case study
The contents marked with an asterisk will be offered as shared
sessions between the Plant and Mammalian Genomics courses.
In four practical exercises complementing the lectures, you will
get familiar with the generation of haploid plant cell cultures,
tracking of gene expression, both quantitatively as well as in
plant tissue, analysis of quantitative trait loci, and genetic
manipulation techniques.
After successful completion of the course, the students will
have gained:
Knowledge
- Describe basic principles for the study of major model plants and
general plant evolution
- Identify and describe the key techniques used for molecular
genetics and selected tools used to analyse genomic information,
including map based cloning and QTLs.
- Explain genetic markers and their use for qualitative and
quantitative traits
- Compare basic central experimental techniques used in plant
genomics and molecular breeding and propose their application for
novel challenges
Skills
- Complete with confidence an assessment of the techniques used to
study complex biological processes in plant model systems
- Apply common bioinformatic tools to screen databases of
model plant species and use genome browsers to analyse simple
problems
- Practice molecular and genetic tools for plant improvement
through molecular breeding of crops for food, fodder and production
of high value crops for e.g. biomedicine, biofuel and green
factories
- Manipulate cell cultures and conduct a simple transformation
procedure
Competences
- Evaluate various forward and reverse genomics approaches for gene
isolation and functional studies
- Relate gene differences with phenotype by means of genomics
- Explain advantages or disadvantages of model plants versus crops
- Communicate effectively with other students in a debate to
justify conclusions on a given problem
Laboratory manuals and a dedicated Plant Genomics Compendium, consisting of all relevant chapters and papers discussed in class will be distributed in electronic form. Next to the compendium, no specific textbook will be required for this course. However, general botany and genetics textbooks may be consulted for the most basic concepts not covered within this course. The student should on his or her own initiative locate additional reading material that matches lecture material.
- Category
- Hours
- Exam
- 4
- Lectures
- 40
- Practical exercises
- 45
- Preparation
- 97
- Theory exercises
- 20
- Total
- 206
As
an exchange, guest and credit student - click here!
Continuing Education - click here!
- Credit
- 7,5 ECTS
- Type of assessment
- Oral examinationOral examination (presentation and questions):
The students receive the assignment at 9.00 and submit an individual, short PowerPoint presentation at 12.00 the next day. The exact date is given in the beginning of the course. At the oral exam the student present the results of the assignment (5 min) followed by a short discussion of the course curriculum (15 min). - Exam registration requirements
Three passed lab reports (75 %) and participation in the oral presentation of case are a requirement
- Aid
- All aids allowed
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examinator
- Re-exam
The lab reports must be handed in two weeks before the re-examination (75% must be approved). Oral examination.
Criteria for exam assesment
See learning outcome.
Course information
- Language
- English
- Course code
- NPLB13001U
- Credit
- 7,5 ECTS
- Level
- Bachelor
- Duration
- 1 block
- Placement
- Block 4
- Schedule
- B
- Course capacity
- No restrictions
- Continuing and further education
- Study board
- Study Board of Natural Resources and Environment
Contracting department
- Department of Plant and Environmental Sciences
Course responsibles
- Thomas Georg Roitsch (7-7b78727d7c6c714979756e7737747e376d74)