NSCPHD1225 Advanced Chromatography and Mass Spectrometry
Advanced course in chromatography and mass spectrometry for chemical analysis of organic compounds. This course is a natural extension of any basic course in analytical chemistry for everybody who is going to use modern hyphenated techniques such as GC-MS, LC-MS(MS) for analysis of complex mixtures. The course covers chromatographic separation of organic compounds with special emphasize on the molecular mechanism and theory of analyte-column interactions for liquid and gas chromatography, and the theory of ionization, fragmentation, mass separation, detection and data-analysis for all kind of mass spectrometers and ionization techniques in common use coupled to gas chromatography, liquid chromatography and capillary electrophoresis. All common analytical instruments and techniques from GC-FID/MS to UPLC-UV/FLU/MSMS are addressed. The theory is supplemented with computer simulations and hands-on experiments and expert guest-lectures of selected topics.
Skills - the students should be able to:
- Describe, compare and contrast, operate and optimise common mass spectrometric ionisation techniques and mass analysers
- Describe, compare and contrast common detectors for chromatography other than mass spectrometers.
- Describe, perform and evaluate fragmentation experiments and exact mass measurements using mass spectrometry.
- Assess, quantify and compensate for matrix effects in ionization processes.
- Describe and use the rate theory, the plate theory, the linear solvent strength model and the hydrophobic subtraction model to optimize chromatography, select and characterize columns and to explain experimental data.
- Describe, compare and contrast, operate common chromatographic techniques.
- Optimise in a time- and cost effective manner any chromatographic separation.
- Critically assess reported chromatographic and/or mass spectrometric research.
- Select a suitable analytical platform for the separation, detection and quantification of analytes from complex matrices including selection and assessment of column, mobile phase and chromatographic parameters, ionisation source and mode, and mass analyser parameterisation.
- Put the chromatographic and mass spectrometric theory and practice into the context of their own, or part of their own, PhD-project research area.
Knowledge - the students should have:
- A solid theoretical understanding of chromatography and mass spectrometry at an advanced level.
Compentences:
- This course enables the student to plan and perform analysis of complex mixtures using modern chromatographic techniques and high-end detectors, including the selection of method, columns and mode of detection, as well as optimization of chromatographic and mass spectrometric conditions for all common instrumental analytical separation methods.
"Introduction to Modern Liquid Chromatography", by Lloyd R. Snyder, Douglas Kirkland and John W. Dolan, Wiley 2009, and "Mass Spectrometry. Principles and Applications", by Edmond de Hoffmann and Vincent Stroobant, 3rd Ed., Wiley 2007. "High-Performance Gradient Elution. The Practical Application of the Linear-Solvent-Strength Model", by Lloyd R. Snyder and John W. Dolan, Wiley 2007 and “Interpretation of Mass Spectra”, by Fred W. McLafferty and Frantisek Turecek, University Science Books 1993 will be used to some extend. Furthermore, selected articles will be included.
For questions feel free to contact Nikoline Juul Nielsen or Bo Svensmark.
- Category
- Hours
- Exam
- 10
- Lectures
- 48
- Practical exercises
- 16
- Preparation
- 120
- Theory exercises
- 16
- Total
- 210
- Credit
- 7,5 ECTS
- Type of assessment
- Oral examination, 30 minThe examination format is a 30 min oral exam with three sub-tasks:
1) An overall subject within e.g. ionization or mass analysers with app. 5 sub-questions for which the student has 1 week of preparation. At the exam the student gives a 5 min introduction and we discuss the subject for an additional 10 min.
2) An overall subject within e.g. chromatographic techniques or detectors for chromatography with app. 2 sub-questions for which the student has 30 min of preparation. At the exam the student gives a short (1-2 min) introduction and we discuss the subject for an additional 5 min.
3) A question within e.g. the linear solvent strength model, the hydrophobic subtraction model or general chromatographic optimization. No preparation and we discuss for 5 min. - Exam registration requirements
- In order to be allowed at the exam 75% of active participation in student colloquia (3 out of4); laboratory exercises (4 out of 5) and handing in approved laboratory reports (4 out of 5) is required, individually or group-wise. The PhD-students should also hand in a project-report in order to get their diploma. 4 ECTS is given if students follow the course in either Chromatography only or Mass Spectrometry only; if this is the case only the subject matter will be assessed at the exam.
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
- Exam period
- Block 2 exam-week, module A exam-day (often Thursday) plus an additional day of the exam-week.
Course information
- Language
- English
- Course code
- NSCPHD1225
- Credit
- 7,5 ECTS
- Level
- Ph.D.
- Duration
- 1 block
- Placement
- Block 2
- Schedule
- A (Tues 8-12 + Thurs 8-17)All Tuesdays and some Thursdays at Campus Frederiksberg. Full day sessions on Thursdays will sometimes be conducted at other research institutions or with industry in the Copenhagen area. If outside Copenhagen area transportation will be organised.
- Course capacity
- Maximum 30 participants. This is the total sum of student allowed from this course or from the master course Advanced Analytical Chemistry – Chromatography and Mass Spectrometry.
- Price
For participants from Danish Universities teaching is free of charge. External students are charged 3000 dkr for the teaching.
All PhD students (including those from Danish Universities) are charged for instrument-time, laboratory expenses and expenses related to excursions amounting to a total of 5000 dkr for the full course (7.5 ECTS), and 3000 dkr for each of the separate parts: Chromatography alone (4 ECTS) or Mass Spectrometry alone (4 ECTS).- Study board
- Natural Sciences PhD Committee
Contracting department
- Department of Plant and Environmental Sciences
Course responsibles
- Nikoline Juul Nielsen (3-7470744676726b7434717b346a71)
- Bo Svensmark (9-75786770756f63746d42726e6770306d7730666d)
Lecturers
Bo Svensmark and Nikoline Juul Nielsen are responsible for the primary part of the teaching; occasionally we also have PhD-students, post docs or senior scientists affiliated in some sense to the Analytical Chemistry Group teach their area of expertise. There is normally an excursion to a company or another research institution, in 2012 and 2013 we visited the national research centre for the working environment (NFA) and where teaching sessions were taught by NFA scientific staff.