NSCPHD1131 ESR Spectroscopy in Food Research

Volume 2014/2015
Content

Aim: To introduce the participants to ESR spectroscopy and radical processes in food in theory and in practice. Contents: General introduction to ESR spectroscopy and instrumentation. Radicals in foods, quantification and characterization. Assays for measuring lipid oxidation and anti-oxidative effects. Testing oxidative stability of foods. Simulation of ESR spectra. Spin trapping and spin probes.
The basic theory behind electron spin resonance spectroscopy and the practical aspects and theory of ESR-instruments will be presented with empasis on subjects that are relevant for food studies. Special emphasis will be put on the use of spin trap, spin label and spin probe techniques, and the concomitant extraction of chemical and physical information from ESR spectra of nitroxyl radicals. The use of ESR in connection with studies of oxidation of foods initiated by metal-containing enzymes will also be covered

Learning Outcome

 

General knowledge about using ESR-spectroscopy, spin trapping, spin probes and spin labels in food systems, and interpretation of spectra.
Practical experience in operating an ESR spectrometer, and recording ESR spectra of food systems.

 

‘Electron Paramagnetic Resonance. A practitioner’s toolkit’ Eds. M. Brustolon & E. Giamello, Wiley 2009.

Lectures, practical exercises and individual practical projects.
The course consists of two parts. Part 1 is an one-week course with lectures and theoretical exercises. Part 1 of the course will be held together with ETH Zürich as a joint winterschool 1. - 6. feb 2015 (http:/​/​www.foodbiochem.ethz.ch/​EPRWinterSchool.html).
Part 2 is a one-week individual practical project with relevance to the students’ PhD-project.
  • Category
  • Hours
  • Lectures
  • 17
  • Practical exercises
  • 16
  • Preparation
  • 45
  • Project work
  • 74
  • Total
  • 152
Credit
6 ECTS
Type of assessment
Written examination
Oral defence
Marking scale
passed/not passed
Censorship form
No external censorship